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RECORD NUMBER: 92 OF 171

Main Title Methods for the Detection of Changes in the Microbial Ecology of Soil Caused by the Introduction of Microorganisms.
Author Doyle, J. D. ; Stotzky, G. ;
CORP Author New York Univ., NY. Dept. of Biology. ;ManTech Environmental Technology, Inc., Corvallis, OR.;Corvallis Environmental Research Lab., OR.
Publisher 1993
Year Published 1993
Report Number EPA-R813650 ;EPA-R-813431; EPA/600/J-93/502;
Stock Number PB94-136157
Additional Subjects Soil microbiology ; Ecology ; Microorganisms ; Respiration ; Carbon dioxide ; Enzymes ; Genetic engineering ; Escherichia coli ; Species diversity ; Antiobiotics ; Measurement ; Changes ;
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NTIS  PB94-136157 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
Abstract Established methods for measuring a broad spectrum of microbial activities were evaluated for their ability to detect perturbations of the soil environment by introduced microorganisms. These methods included: gross metabolic activity and carbon mineralization, as measured by respiration (CO2 evolution); activity of selected enzymes (arylsulfatases, acid and alkaline phosphatases, dehydrogenases); and indicators of the community structure of the indigenous microbiota (species diversity, physiological groups, antibiotic-resistance profiles). The evaluation of these variables, as well as the fate of the introduced microorganisms (Escherichia coli W3110 (R702) and its homologous, plasmidless, parental strain, W3110), was conducted over a 33-day period. Statistically significant differences only in some components of the microbiota were detected between soil inoculated with W3110 (R702) or W3110 of not inoculated. However, these differences were transient and, therefore, probably not ecologically significant.
Supplementary Notes Pub. in Microbe Releases 2, p63-72 1993. See also PB91-219022. Prepared in cooperation with ManTech Environmental Technology, Inc., Corvallis, OR. Sponsored by Corvallis Environmental Research Lab., OR.
NTIS Title Notes Journal article.
Category Codes 48E; 57K; 57H
NTIS Prices PC A03/MF A01
Primary Description 600/02
Document Type NT
Cataloging Source NTIS/MT
Control Number 409118146
Origin NTIS
Type CAT