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Main Title Survival and Effects of Wild-Type, Mutant, and Recombinant 'Streptomyces' in a Soil Ecosystem.
Author Wang, Z. ; Crawford, D. L. ; Pometto, A. L. ; Rafii., F. ;
CORP Author Idaho Univ., Moscow. Dept. of Bacteriology and Biochemistry. ;Iowa State Univ., Ames. ;Idaho Agricultural Experiment Station, Moscow.;Corvallis Environmental Research Lab., OR.
Publisher c1989
Year Published 1989
Report Number EPA-R-813486; EPA/600/J-89/541;
Stock Number PB91-231258
Additional Subjects Terrestrial ecosystems ; Soil microbiology ; Streptomyces ; Mutation ; Genetic recombination ; Survival analysis ; Plasmids ; Genetic engineering ; Lignin ; Microbial colony count ; Minerals ; Soil chemistry ; Carbon dioxide ; Bacterial spores ; Reprints ;
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NTIS  PB91-231258 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
Abstract In a laboratory simulation, selected wild-type, mutant, and recombinant Streptomyces were released into a silt loam soil. Strains included genetically enhanced lignin decomposers and those expressing recombinant plasmids. Their survival and effects on soil organic carbon mineralization were monitored in sterile and non-sterile soil, with and without lignocellulose supplementation. Survival was followed by viable plate counts on selective media. This is the first report of released, genetically altered Streptomyces having a measurable effect on a natural ecosystem.
Supplementary Notes Pub. in Canadian Jnl. of Microbiology, v35 p535-543 1989. Also pub. as Idaho Agricultural Experiment Station, Moscow. paper no. 8951. Prepared in cooperation with Iowa State Univ., Ames, and Idaho Agricultural Experiment Station, Moscow. Sponsored by Corvallis Environmental Research Lab., OR.
NTIS Title Notes Journal article.
Title Annotations Reprint: Survival and Effects of Wild-Type, Mutant, and Recombinant 'Streptomyces' in a Soil Ecosystem.
Category Codes 57K; 57F
NTIS Prices PC A03/MF A01
Primary Description 600/02
Document Type NT
Cataloging Source NTIS/MT
Control Number 128229503
Origin NTIS
Type CAT