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Main Title Improved Flotation Technique for Microscopy of 'In situ' Soil and Sediment Microorganisms.
Author Bone, T. L. ; Balkwill, D. L. ;
CORP Author Florida State Univ., Tallahassee. Dept. of Biological Science.;Robert S. Kerr Environmental Research Lab., Ada, OK.
Year Published 1986
Report Number EPA/600/J-86/031;
Stock Number PB86-190139
Additional Subjects Flotation ; Sediments ; Microorganisms ; Aquifers ; Tests ; Electron microscopy ; Saturated soils ; Morphology ; Sampling ; Cells(Biology) ; Viability ; Populations ; Growth ; Recovery ; Reprints ; Species diversity
Library Call Number Additional Info Location Last
NTIS  PB86-190139 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
Abstract An improved flotation method for microscopical examination of in situ soil and sediment microorganisms was developed. Microbial cells were released into gel-like flotation films that were stripped from soil and sediment aggregates as these aggregates were submerged in 0.5% solutions of poly-vinylpryrrolidone (PVP). The use of PVP solutions instead of water facilitated the release of films from saturated samples like aquifer sediments, as well as from typical surface soils. In situ microbial morphological characteristics could then be surveyed rapidly by light microscopical examination of films stained with acridine orange. The method effectively determined the range of morphological diversity in a variety of sample types. (Copyright (c) 1986, American Society for Microbiology.)
Supplementary Notes Pub. in Applied and Environmental Microbiology, v51 n3 p462-468 Mar 86. Sponsored by Robert S. Kerr Environmental Research Lab., Ada, OK.
NTIS Title Notes Journal article.
Title Annotations Reprint: Improved Flotation Technique for Microscopy of 'In situ' Soil and Sediment Microorganisms.
PUB Date Free Form c1986
Category Codes 8H; 8M; 6M; 68D; 48G; 48E; 57K
NTIS Prices PC A02/MF A01
Primary Description 600/15
Document Type NT
Cataloging Source NTIS/MT
Control Number 618818305
Origin NTIS
Type CAT