| Main Title |
L5178 TK +/- Mouse Lymphoma Mutagenesis Assay of 2,2,4-Trimethylpentane with Cover Letter dated 07/10/87. |
| CORP Author |
American Petroleum Inst., Washington, DC.;Environmental Protection Agency, Washington, DC. Office of Toxic Substances. |
| Publisher |
23 Jul 1987 |
| Year Published |
1987 |
| Report Number |
FYI-AX-0787-0560; |
| Stock Number |
OTS-0000560-0 |
| Additional Subjects |
Toxicity ;
Sampling ;
Procedures ;
Documentation ;
Concentrations ;
Equipment ;
Rats ;
Laboratory animals ;
Mouse lymphoma mutagenesis assay ;
2,2,4-Trimethylpentane ;
Aroclor ;
American Petroleum Institute
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
OTS-0000560-0 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
108p |
| Abstract |
American Petroleum Institute's test article 2,2,4- Trimethlpentane (MBA no. T5316) was tested in the L5178y TX+/- Mouse Lymphoma Mutagenesis Assay in the presence and absence of Aroclor induced rat liver S9. In the first assay, the nonactivated cultures were not in the 10% to 50% Total Growth range. Several critical S9 activated cultures were contaminated and the trial was repeated. In the second assay, the nonactivated solvent control cultures produced unacceptably high background mutant frequencies. The S9 activated cultures in this assay were too toxic to clone. In the third assay, the nonactivated cultures that were cloned had been treated with a range of test article concentrations from 3.0 microl/ml to 0.5 microl/ml. These concentrations produced a range in Total Growth of 6% to 61%. The S9 activated cultures that were cloned had been treated with a range of test article concentrations from 2.5 microl/ml to 0.5 microl/ml. These concentrations produced from 36% to 91% Total Growth. Two of the nonactivated cultures that were cloned exhibited mutant frequencies which were at least twice the mean mutant frequency of the solvent controls. The viable counts of these cultures were low due to poor cloning efficiencies thus producing high mutant frequencies. |
| Supplementary Notes |
The quality of the documents listed in the Office of Toxic Substances database may not meet usual NTIS standards but are included to further the opportunity for the scientific and technical community to locate materials which may not be available elsewhere. The content is the responsibility of OTS. Prepared in cooperation with American Petroleum Inst., Washington, DC. |
| Availability Notes |
Product reproduced from digital image. Order this product from NTIS by: phone at 1-800-553-NTIS (U.S. customers); (703)605-6000 (other countries); fax at (703)605-6900; and email at orders@ntis.gov. NTIS is located at 5285 Port Royal Road, Springfield, VA, 22161, USA. |
| Category Codes |
57Y; 57O |
| NTIS Prices |
PC A07/MF$25.00 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
019101070 |
| Origin |
NTIS |
| Type |
CAT |
