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RECORD NUMBER: 500 OF 1139

Main Title Potential for Transduction of Plasmids in a Natural Freshwater Environment: Effect of Plasmid Donor Concentration and a Natural Microbial Community on Transduction in 'Pseudomonas aeruginosa'.
Author Saye, D. J. ; Ogunseitan, O. ; Sayler, G. S. ; Miller, R. V. ;
CORP Author Stritch School of Medicine, Maywood, IL. Dept. of Biochemistry and Biophysics. ;Tennessee Univ., Knoxville. Dept. of Microbiology.;Environmental Research Lab., Gulf Breeze, FL.
Year Published 1987
Report Number EPA/600/J-87/145;
Stock Number PB88-153820
Additional Subjects Pseudomonas ; Fresh water biology ; Reservoirs ; Deoxyribonucleic acids ; Concentration(Composition) ; Genetics ; Genes ; Bacteria ; Culture media ; Environmental tests ; Experimental data ; Models ; Graphs(Charts) ; Reprints ; Habitats ; Pseudomonas aeruginosa ; Transduction ; Plasmids
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NTIS  PB88-153820 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
Abstract The transduction of Pseudomonas aeruginosa plasmid Rms149 by the generalized transducing phage DS1 was shown to occur during a nine day incubation of environmental test chambers in a freshwater reservoir. Plasmid DNA was transferred from a nonlysogenic plasmid donor to a DS1 lysogen of Pseudomonas aeruginosa that served both as the source of the transducing phage and as the recipient of the plasmid DNA. Transduction of the plasmid in the presence of the natural microbial community of the reservoir was below the limits of detection employed. The results demonstrate that a potential exists for the transduction of plasmid DNA in aquatic habitats. (Copyright (c) 1987, American Society for Microbiology.)
Supplementary Notes Pub. in Applied and Environmental Microbiology, v53 n5 p987-995 May 87. Prepared in cooperation with Tennessee Univ., Knoxville. Dept. of Microbiology. Sponsored by Environmental Research Lab., Gulf Breeze, FL.
NTIS Title Notes Journal article.
Title Annotations Reprint: Potential for Transduction of Plasmids in a Natural Freshwater Environment: Effect of Plasmid Donor Concentration and a Natural Microbial Community on Transduction in 'Pseudomonas aeruginosa'.
PUB Date Free Form c1987
Category Codes 57; 57K
NTIS Prices PC A03/MF A01
Primary Description 600/04
Document Type NT
Cataloging Source NTIS/MT
Control Number 808422550
Origin NTIS
Type CAT