| Main Title |
Quantitative Dot-Immunobinding Assay for Proteins Using Nitrocellulose Membrane Filters. |
| Author |
Jahn, R. ;
Schiebler, W. ;
Greengard, P. ;
|
| CORP Author |
Rockefeller Univ., New York.;Health Effects Research Lab., Research Triangle Park, NC. |
| Year Published |
1984 |
| Report Number |
EPA-R-810608; EPA/600/J-84/382; |
| Stock Number |
PB87-146205 |
| Additional Subjects |
Meetings ;
Protein ;
Quantitative analysis ;
Rats ;
Brain ;
Immunoassay
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB87-146205 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
7p |
| Abstract |
An immunoassay method is described for the quantitative determination of synapsin I (protein I) and of a 36,000-dalton membrane protein from rat brain synaptic vesicles. The samples are spotted on nitrocellulose membrane filters, incubated sequentially with specific antibodies and -125 I-labeled protein A, and assayed for radioactivity in a gamma scintillation counter. Conditions have been established to prevent losses of protein from the sheets during processing, to quench background radioactivity, and to adjust the sensitivity to the range desired. A large number of samples can be handled in parallel. The assay does not require iodination of the antigen and is accurate even with crude tissue samples. Standard curves were linear over a 20- to 50-fold range. The sensitivity of the method is such that 10 pmol of synapsin I and 50 ng of total vesicle membrane protein could be measured with accuracy. The method should prove useful for a wide range of proteins. |
| Supplementary Notes |
Pub. in Proceedings of the National Academy of Science, v81 p1684-1687 Mar 84. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| PUB Date Free Form |
c1984 |
| Category Codes |
57B |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
709318922 |
| Origin |
NTIS |
| Type |
CAT |
