| Main Title |
Modulation of eicosanoid production by human alveolar macrophases exposed to silica 'in vitro'. {microfiche} |
| Author |
Koren, H.S. ;
Joyce, M. ;
Devlin, R. B. ;
Becker, S. ;
Driscoll., K.
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;North Carolina Univ. at Chapel Hill. School of Medicine. ;Procter and Gamble Co., Cincinnati, OH. Miami Valley Labs. ;ABB Environmental, Inc., Chapel Hill, NC. |
| Publisher |
US Environmental Protection Agency, Health Affects Research Laboratory, |
| Year Published |
1991 |
| Report Number |
EPA/600/D-90/196 |
| Stock Number |
PB91-136630 |
| Additional Subjects |
Silicon organic compounds ;
Toxicity ;
Dust ;
Particles ;
Respiration ;
In vitro analysis ;
Liquid chromatography ;
Inflammation ;
Lung ;
Humans ;
Laboratory animals ;
Pulmonary alveoli ;
Macrophages ;
Eicosanoids ;
Dose-response relationships ;
Bronchoalveolar lavage fluid ;
Lipoxygenases ;
Prostaglandin synthase ;
Cell survival ;
Leukotrienes ;
Radioimmunoassay
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB91-136630 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
30 p. : ill. ; 29 cm. |
| Abstract |
The objective of the study was to determine the eicosanoid production by human alveolar macrophages in response to silica exposure in vitro and to assess their contribution to silica-induced fibrosis and inflammation. Macrophages were obtained from healthy volunteers and were incubated for 3 or 24 hours in the presence of silica (100, 60, and 0 microgram/ml). Supernatants were removed for eicosanoid analysis. Eicosanoids were analyzed by both HPLC and RIA. The data suggested that silica caused an increased release of LTB4, LTC4/D4/E4, and 5-HETE after 3 hours; and decreases in PGE2 and TXB2 production after 24 hours exposure to 100 microgram/ml silica. In addition, 12-HETE and 15-HETE production remained unchanged at either time point. These opposing effects seen with the metabolites of lipoxygenase and cyclooxygenase pathways could contribute to silica-induced fibrosis. The pattern of eicosanoid production after exposure to silica was different from that obtained when macrophages were stimulated with LPS for 3 or 24 hrs, indicating that the response to the particles was not just due to general cellular activation. |
| Notes |
"EPA 600/D-90/196." |
| Place Published |
Research Triangle Park, NC., |
| Supplementary Notes |
Prepared in cooperation with North Carolina Univ. at Chapel Hill. School of Medicine, Procter and Gamble Co., Cincinnati, OH. Miami Valley Labs., and ABB Environmental, Inc., Chapel Hill, NC. |
| PUB Date Free Form |
1990. |
| NTIS Prices |
PC A03/MF A01 |
| BIB Level |
m |
| Cataloging Source |
OCLC/T |
| OCLC Time Stamp |
20001226165342 |
| Language |
eng |
| Origin |
NTIS |
| Type |
MERGE |
| OCLC Rec Leader |
00813nam 2200205Ka 45020 |
