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RECORD NUMBER: 239 OF 321

Main Title Reduction of Interfering Cytotoxicity Associated with Wastewater Sludge Concentrates Assayed for Indigenous Enteric Viruses.
Author Hurst, C. J. ; Goyke, T. ;
CORP Author Environmental Monitoring and Support Lab.-Cincinnati, OH.
Year Published 1983
Report Number EPA-600/J-83-083;
Stock Number PB84-116524
Additional Subjects Viruses ; Public health ; Solid waste disposal ; Toxicity ; Chemical analysis ; Adsorption ; Flocculation ; Sampling ; Precipitation(Chemistry) ; Extraction ; Washing ; Polyelectrolytes ; Reprints ; Sewage sludges Cytotoxicity ; Beef extract
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NTIS  PB84-116524 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
Abstract Washing, freon extraction, and cationic polyelectrolyte precipitation were compared for their ability to reduce cytotoxicity associated with virus concentrates derived from beef extract eluates of wastewater sludges. Eluates concentrated by hydroextraction were usually much more toxic than those concentrated by organic flocculation. This difference may be due entirely to nondialyzable material naturally present in the beef extract which did not precipitate during flocculation at pH 3.5. Washing inoculated cell monolayers with saline containing calf serum before the addition of agar overlay media was most effective in reducing cytotoxicity, although it resulted in a greater virus loss, as compared with freon extraction and cationic polyelectrolyte precipitation. (Copyright (c) 1983, American Society for Microbiology.)
Availability Notes Pub. in Applied and Environmental Microbiology, v46 n1 p133-139 Jul 83.
NTIS Title Notes Journal article.
Title Annotations Reprint: Reduction of Interfering Cytotoxicity Associated with Wastewater Sludge Concentrates Assayed for Indigenous Enteric Viruses.
PUB Date Free Form c1983
Category Codes 6T; 7D; 6E; 68G; 68C; 57Y; 99A; 57U
NTIS Prices Not available NTIS
Primary Description 600/06
Document Type NT
Cataloging Source NTIS/MT
Control Number 403420995
Origin NTIS
Type CAT