| Main Title |
Application of DNA-DNA Colony Hybridization to the Detection of Catabolic Genotypes in Environmental Samples. |
| Author |
Sayler, G. S. ;
Shields, M. S. ;
Tedford, E. T. ;
Breen, A. ;
Hooper, S. W. ;
|
| CORP Author |
Tennessee Univ., Knoxville.;Robert S. Kerr Environmental Research Lab., Ada, OK. |
| Year Published |
1985 |
| Report Number |
EPA/600/J-85/116; |
| Stock Number |
PB86-101839 |
| Additional Subjects |
Bacteria ;
Toluene ;
Naphthalene ;
Aromatic hydrocarbons ;
DNA ;
Hybridization ;
Chemical attack(Degradation) ;
Environmental microbiology ;
Molecular cloning ;
Environmental pollutants ;
Plasmids
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB86-101839 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
10p |
| Abstract |
The application of preexisting DNA hybridization techniques was investigated for potential in determining populations of specific gene sequences in environmental samples. Cross-hybridizations among two degradative plasmids, TOL and NAH, and two cloning vehicles, pLAFRI and RSF1010, were determined. The detection limits for the TOL plasmid against a nonhomologous plasmid-bearing bacterial background was ascertained. |
| Availability Notes |
Pub. in Applied and Environmental Microbiology, v49 n5 p1295-1303 May 85. |
| NTIS Title Notes |
Journal article. |
| PUB Date Free Form |
c1985 |
| Category Codes |
6F; 6M; 57K; 57H; 57F |
| NTIS Prices |
Not available NTIS |
| Primary Description |
600/15 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
535324861 |
| Origin |
NTIS |
| Type |
CAT |
