Main Title |
Sequential Inoculation as an Adjunct in Enteric Virus Plaque Enumeration. |
Author |
Benton, W. H. ;
Hurst., C. J. ;
|
CORP Author |
Health Effects Research Lab., Cincinnati, OH. Toxicology and Microbiology Div. |
Publisher |
c1990 |
Year Published |
1990 |
Report Number |
EPA/600/J-90/230; |
Stock Number |
PB91-117085 |
Additional Subjects |
Inoculation ;
Sewage ;
Reprints ;
Plaque assay ;
Enteric viruses ;
Cell line ;
Idoxuridine ;
Echoviruses ;
Poliovirus
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB91-117085 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
7p |
Abstract |
The potential utility of sequentially inoculating a virus sample onto two different cultures of similar vs dissimilar cell lines was evaluated in conjunction with IDU (5-iodo-2'-deoxyuridine) treatment of the cells as a potential adjunct in viral plaque formation assays. This evaluation was done using laboratory grown human echovirus 7, human enterovirus 69 and human poliovirus 1, plus an environmental concentrate derived from sewage that contained indigenous untyped enteroviruses. The cell lines employed were BGM, RD, L-132 and HEL-299. Sequential inoculation generally yielded higher viral assay titers when compared with the more traditional method of simply introducing viral inoculum onto a culture of the first (initial) cell line and then completing the assay without removing that inoculum. When a permissive cell line (BGM or RD) was used for both the initial and final cultures in a sequential inoculation technique, the total plaque count titer from both the initial plus final cultures represented an average 35% improvement over the traditional method. |
Supplementary Notes |
Pub. in Water Research, v24 n7 p905-909 Jul 90. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Sequential Inoculation as an Adjunct in Enteric Virus Plaque Enumeration. |
Category Codes |
57K |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
101123892 |
Origin |
NTIS |
Type |
CAT |