Full Record Display for the EPA National Library Catalog

RECORD NUMBER: 8 OF 18

OLS Field Name OLS Field Data
Main Title Evaluation of Mixed Cell Types and 5-Iodo-2'-Deoxyuridine Treatment upon Plaque Assay Titers of Human Enteric Viruses (Journal Version).
Author Benton, W. H. ; Hurst, C. J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Publisher 1986
Year Published 1986
Report Number EPA/600/J-86/474;
Stock Number PB89-106892
Additional Subjects Enteroviruses ; Idoxuridine ; Plaque assay ; Cell lines ; Echoviruses ; Coxsackieviruses ; Human adenoviruses ; Iodine organic compounds ; Nucleosides ; Poliovirus ; Viral cytopathogenic effect ; Water microbiology ; Reprints ; Virus isolation
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB89-106892 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 03/14/1989
Collation 6p
Abstract Four continuous cell lines BGM, L-132, HEL-299, and RD were compared both when cultured separately and as mixtures for use in plaque assay titrations of human Adenovirus 1 and six human enterovirus serotypes. The effect of incubating these cell cultures in media containing IDU (5-iododeoxyuridine) prior to inoculation with virus was also studied. The use of mixed cell cultures revealed cell line dependent synergistic as well as inhibitory effects. These effects were strongly virus dependent. The effect of IDU pretreatment was also virus and cell line specific and enabled some viruses to form plaques in cell lines when they otherwise would not. Overall, IDU pretreatment resulted in an approximate two-fold increase in plaque titers over those obtained without treatment.
Supplementary Notes Pub. in Applied and Environmental Microbiology, v51 n5 p1036-1040 May 86.
NTIS Title Notes Journal article.
Title Annotations Reprint: Evaluation of Mixed Cell Types and 5-Iodo-2'-Deoxyuridine Treatment upon Plaque Assay Titers of Human Enteric Viruses (Journal Version).
Category Codes 57K; 57U; 68D; 68G
NTIS Prices PC A02/MF A01
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 834422663
Origin NTIS
Type CAT