Full Record Display for the EPA National Library Catalog
RECORD NUMBER: 3 OF 25| Main Title | Biodegradation of Crystal Violet by the White Rot Fungus 'Phanerochaete chrysosporium'. | |||||||||||
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| Author | Bumpus, J. A. ; Brock, B. J. ; | |||||||||||
| CORP Author | Utah State Univ., Logan.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. | |||||||||||
| Publisher | c1988 | |||||||||||
| Year Published | 1988 | |||||||||||
| Report Number | EPA/600/J-93/163; | |||||||||||
| Stock Number | PB93-191609 | |||||||||||
| Additional Subjects | Gentian violet ; Biodeterioration ; Dyes ; Hydrogen peroxide ; Mass spectrometry ; Metabolism ; Lignin ; High pressure liquid chromatography ; Reprints ; Phanerochaete chrysosporium ; Lignin peroxidase | |||||||||||
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| Collation | 10p | |||||||||||
| Abstract | Biodegradation of crystal violet (N,N,N',N',N',N''- hexamethylpararosaniline) in ligninolytic (nitrogen-limited) cultures of the white rot fungus Phanerochaete chrysosporium was demonstrated by the disappearance of crystal violet and by the identification of three metabolites (N,N,N',N',N'' -pentamethylpararosaniline, N,N,N',N'' -tetramethylpararosaniline, and N,N',N'' -trimethylpararosaniline) formed by sequential N-demethylation of the parent compound. Metabolite formation also occurred when crystal violet was incubated with the extracellular fluid obtained from ligninolytic cultures of this fungus, provided that an H2O2-generating system was supplied. This, as well as the fact that a purified ligninase catalyzed N-demethylation of crystal violet, demonstrated that biodegradation of crystal violet by this fungus is dependent, at least in part, upon its lignin-degrading system. In addition to crystal violet, six other triphenylmethane dyes (pararosaniline, cresol red, bromphenol blue, ethyl violet, malachite green, and brilliant green) were shown to be degraded by the lignin-degrading system of this fungus. (Copyright (c) 1988 American Society for Microbiology.) | |||||||||||
| Supplementary Notes | Pub. in Applied and Environmental Microbiology, v54 n5 p1143-1150 May 88. See also PB93-191617. Sponsored by Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. | |||||||||||
| NTIS Title Notes | Journal article. | |||||||||||
| Title Annotations | Reprint: Biodegradation of Crystal Violet by the White Rot Fungus 'Phanerochaete chrysosporium'. | |||||||||||
| Category Codes | 57K; 57B | |||||||||||
| NTIS Prices | PC A02/MF A01 | |||||||||||
| Primary Description | 600/14 | |||||||||||
| Document Type | NT | |||||||||||
| Cataloging Source | NTIS/MT | |||||||||||
| Control Number | 318931297 | |||||||||||
| Origin | NTIS | |||||||||||
| Type | CAT |