Full Record Display for the EPA National Library Catalog


Main Title Biological Oxidations of Organic Compounds by Enzymes from a White Rot Fungus.
Author Bumpus, J. A. ; Mileski, G. ; Brock, B. ; Ashbaugh, W. ; Aust, S. D. ;
CORP Author Utah State Univ., Logan. Biotechnology Center.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher 1993
Year Published 1993
Report Number EPA/600/A-93/100;
Stock Number PB93-191369
Additional Subjects Biodeterioration ; Oxidation ; Organic compounds ; Waste treatment ; High pressure liquid chromatography ; Reprints ; Phanerochaete chrysosporium ; Ligninases
Library Call Number Additional Info Location Last
NTIS  PB93-191369 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
Abstract The ability of the white rot fungus Phanerochaete chrysosporium to degrade a wide variety of structurally diverse organopollutants is dependent upon the lignin-degrading system of the microorganism. Current research is focused on ways to increase ligninase activity in culture and on understanding the enzymology of the system as it affects xenobiotic oxidation. Ligninase activity is expressed by the fungus in response to nutrient nitrogen limiting conditions. In agitated cultures (1 L total volume in 2.8 L Fernbach flasks), ligninase activities up to 446 units (pmoles of veratryl alcohol oxidized) per liter, representing approximately a 20-fold increase over standard stationary cultures, were observed. Furthermore, ligninase activity was shown to be cyclic, reaching peak activities at about 30-hour intervals. Substantial ligninase activity (200 units/liter) was also observed in 100-mL stationary cultures. In these cultures, veratryl alcohol may serve to induce ligninase biosynthesis, and/or it may protect the enzyme from subsequent inactivation.
Supplementary Notes Pub. in Biological Processes. Innovative Hazardous Waste Treatment Technology Series, v3 p47-54 1991. See also PB93-191351. Sponsored by Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
NTIS Title Notes Journal article.
Title Annotations Reprint: Biological Oxidations of Organic Compounds by Enzymes from a White Rot Fungus.
Category Codes 57K; 57B; 57F; 68
NTIS Prices PC A02/MF A01
Primary Description 600/14
Document Type NT
Cataloging Source NTIS/MT
Control Number 320425599
Origin NTIS
Type CAT