||Biodegradation of 2,4,5-Trichlorophenoxyacetic Acid in Liquid Culture and in Soil by the White Rot Fungus 'Phanerochaete chrysosporium'.
Ryan, T. P. ;
Bumpus, J. A. ;
||Utah State Univ., Logan.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.;National Inst. of Environmental Health Sciences, Research Triangle Park, NC.
||EPA-R-813369 ;EPA-R-814448; EPA/600/J-93/157;
Soil contamination ;
Chemical water pollutants ;
Carbon 14 ;
Culture media ;
Soil microbiology ;
Thin layer chromatography ;
High pressure liquid chromatography ;
Carbon dioxide ;
Phanerochaete chrysosporium ;
||Some EPA libraries have a fiche copy filed under the call number shown.
||Extensive biodegradation of (14)C-2,4,5-trichlorophenoxyacetic acid ((14)C-2,4,5-T) by the white rot fungus Phanerochaete chrysosporium was demonstrated in nutrient nitrogen-limited aqueous cultures and in (14)C-2,4,5-T-contaminated soil inoculated with this fungus and supplemented with ground corn cobs. After incubation of (14)C-2,4,5-T with aqueous cultures of the fungus for 30 days, 62.0% - or + 2.0% of the (14)C-2,4,5-T initially present was degraded to (14)CO2. Mass balance analysis demonstrated that water soluble metabolites were formed during degradation, and HPLC and thin layer chromatography (TLC) of methylene chloride-extractable material revealed the presence of polar and non-polar (14)C-2,4,5-T initially present in cultures remained as undegraded (14)C-2,4,5-T. In incubations composed of (14)C-2,4,5-T-contaminated soil, ground corn cobs, and 40% (w/w) water, 32.5%-3.6% of the (14)C-2,4,5-T initially present was converted to (14)CO2 after 30 days of incubation. These results suggest that it may be possible to develop practical systems based on the use of the fungus to detoxify 2,4,5-T-contaminated water and soil. (Copyright (c) Springer-Verlag 1989.)
||Pub. in Applied Microbiology and Biotechnology, v31 p302-307 1989. See also PB93-191567. Sponsored by Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab., and National Inst. of Environmental Health Sciences, Research Triangle Park, NC.
|NTIS Title Notes
||Reprint: Biodegradation of 2,4,5-Trichlorophenoxyacetic Acid in Liquid Culture and in Soil by the White Rot Fungus 'Phanerochaete chrysosporium'.
||57K; 57B; 68D; 68C; 48E
||PC A02/MF A01