| Main Title |
Effects of Selected Neuroactive Chemicals on Calcium Transporting Systems in Rat Cerebellum and on Survival of Cerebellar Granule Cells. |
| Author |
Kodavanti, P. R. S. ;
Mundy, W. R. ;
Tilson, H. A. ;
Harry, G. J. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div. ;National Inst. of Environmental Health Sciences, Research Triangle Park, NC. Div. of Intramural Research. |
| Publisher |
18 Jun 93 |
| Year Published |
1993 |
| Report Number |
EPA/600/J-94/071; |
| Stock Number |
PB94-141595 |
| Additional Subjects |
Cerebellum ;
Toxic substances ;
Nervous system ;
Calcium ;
Rats ;
Biological transport ;
Ca(2+)-transporting ATPase ;
Cell survival ;
Lactate dehydrogenase ;
Aluminum ;
N-methyl aspartate ;
Mitochondria ;
Microsomes ;
Chlorpromazine ;
Reprints ;
Permethrin ;
Deltamethrin
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB94-141595 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
11p |
| Abstract |
This investigation examined the relationship between alteration of Ca(2+)-transport systems and cytotoxicity in vitro for a number of neuroactive chemicals including environmental pollutants. (45)Ca(2+) uptake as a measure of Ca(2+) sequestration was determined in mitochondria and microsomes isolated from cerebella of adult male Long-Evans hooded rats by differential centrifugation. Ca(2+) extrusion, measured as Ca(2+)-ATPase activity, was determined in synaptosomes prepared by sucrose density gradient. Cytotoxicity (lactate dehydrogenase leakage) was assessed in primary cultures of cerebellar granule cells from 6- to 8-day old Long-Evans rats. N-Methyl-D-aspartic acid (NMDA) did not alter synaptosomal Ca(2+)-ATPase activity or (45)Ca(2+) uptake in mitochondria and microsomes. However, chlorpromazine (CPZ), aluminum (Al), permethrin (PER), and deltamethrin (DEL) inhibited Ca(2+) sequestration by mitochondria and microsomes. Of all the chemicals tested, CPZ was the most potent in inhibiting Ca(2+)-transporting systems and was also cytotoxic. |
| Supplementary Notes |
Pub. in Fundamental and Applied Toxicology, v21 n3 p308-316 Oct 93. Presented at the Annual Meeting of Society of Toxicology (31st), Seattle, WA., February 1992. Prepared in cooperation with National Inst. of Environmental Health Sciences, Research Triangle Park, NC. Div. of Intramural Research. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Effects of Selected Neuroactive Chemicals on Calcium Transporting Systems in Rat Cerebellum and on Survival of Cerebellar Granule Cells. |
| Category Codes |
57Y; 57F; 57S |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
407722638 |
| Origin |
NTIS |
| Type |
CAT |
