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Main Title

Guidelines for conducting early life stage toxicity tests with Japanese medaka (Oryzias latipes) /

Author

Benoit, Duane A. ; Benoit, D. A. ; Holcombe, G. W. ; Spehar, R. L.

Other Authors

Author	Title of a Work
Holcombe, Gary W.
Spehar, Robert L.

CORP Author

Environmental Research Lab.-Duluth, MN.

Publisher

U.S. Environmental Protection Agency,

Year Published

1991

Report Number

EPA/600/3-91/063

Stock Number

PB92-137488

OCLC Number

25110349

Subjects

Oryzias latipes ; Toxicity testing

Additional Subjects

Water pollution effects(Animals) ; Oryzias ; Life cycles ; Toxic substances ; Embryo ; Test methods ; Manuals ; Temperature ; Exposure ; Light ; Oxygen ; Guidelines

Internet Access

Description	Access URL
	https://nepis.epa.gov/Exe/ZyPDF.cgi?Dockey=30000OX0.PDF

Holdings

Library	Call Number	Additional Info	Location	Last Modified	Checkout Status
EHBD	EPA/600/3-91/063		CEMM/ACESD Library/Narragansett,RI	03/16/2007
EJED	EPA-600/3-91-063		OCSPP Chemical Library/Washington,DC	07/24/1992
EKCD	EPA/600/3-91/063		CEMM/GEMMD Library/Gulf Breeze,FL	07/17/2018
ELBD ARCHIVE	EPA 600-3-91-063	Received from HQ	AWBERC Library/Cincinnati,OH	10/04/2023	DISPERSAL
ELDD	EPA/600/3-91/063		CCTE/GLTED Library/Duluth,MN	05/30/1992
EMBD	EPA/600/3-91/063		NRMRL/GWERD Library/Ada,OK	05/01/1992
ERAD	EPA 600/3-91/063		Region 9 Library/San Francisco,CA	11/27/1992
ESAD	EPA 600-3-91-063		Region 10 Library/Seattle,WA	03/23/2010
NTIS	PB92-137488	Some EPA libraries have a fiche copy filed under the call number shown.		07/26/2022

Collation

25 pages : illustrations ; 28 cm

Abstract

The manual represents a procedural guide for conducting embryo-larval early life stage (ELS) toxicity tests with Japanese medaka (Oryzias latipes). These procedures are based upon evaluation of published papers and recent methods development work conducted at a laboratory in Duluth. The published papers are referenced in the appropriate places throughout the text of the report. If more detailed information on test apparatus or specific biological and chemical methods is desired, the reader is encouraged to study the reference material or contact one of the authors of the manual. All routine methods not covered in the procedure (physical and chemical determinations), should be followed as described in Standard Methods for the Examination of Water and Wastewater (3). It is suggested that those routines dealing with handling of fish not covered in the procedure should be followed as described by ASTM (4).

Notes

"EPA/600/3-91/063." "December 1991." "Environmental Research Laboratory-Duluth, Office of Research and Development." Includes bibliographical references.

Place Published

Duluth, MN

Supplementary Notes