||Neutrophil Myeloperoxidase Destruction by Ultraviolet Irradiation.
Hanker, J. ;
Giammara, B. ;
Strauss, G. ;
||North Carolina Univ. at Chapel Hill. ;Louisville Univ., KY.;Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.
Ultraviolet radiation ;
PUVA therapy ;
Scanning electron microscopy
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||The peroxidase activity of enriched leukocyte preparations on coverslips was determined cytochemically with a newly developed method. The techniques utilizes diaminobenzidine medium and cupric nitrate intensification and is suitable for analysis with light microscopy, SEM, and TEM. Blood specimens from control individuals were studied with and without in vitro UV irradiation and compared with those from psoriasis patients exposed therapeutically to various types of UV in phototherapy. All UV irradiated samples showed diminished neutrophil myeloperoxidase (MP) activity although that of the principal eosinophil peroxidase was unaffected. The SEMs supported the contention that decreased neutrophil MP activity might be related to UV induced degranulation. It is believed to be possible, eventually, to equate the observed MP degranulation effect after UV irradiation with diminished ability to fight bacterial infections. (Copyright (c) 1988 by San Francisco Press, Inc.)
||Pub. in Jnl. of Electron Microscopy, p138-139 Jun 88. Prepared in cooperation with Louisville Univ., KY. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.
|NTIS Title Notes
||Reprint: Neutrophil Myeloperoxidase Destruction by Ultraviolet Irradiation.
||PC A01/MF A01