Full Record Display for the EPA National Library Catalog

RECORD NUMBER: 222 OF 286

Main Title Mutational Spectrum and Recombinogenic Effects Induced by Aminofluorene Adducts in Bacteriophage M13 (Journal Version).
Author Ross, J. ; Doisy, R. ; Tang, M. S. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Texas Univ. System Cancer Center, Smithville.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/190;
Stock Number PB89-119788
Additional Subjects Coliphages ; Mutations ; Genetic recombination ; Fluorenes ; Carcinogens ; Bacteriophages ; Amines ; DNA mutational analysis ; Reprints ; Aminofluorenes ; Fluorene/amino-N-hydroxy
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB89-119788 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
Abstract Double stranded replicative form (RFI) DNA of bacteriophage M13mp10 has been modified in vitro to various extents with N-hydroxy-2-aminofluorene (N-OH-AF) and then transfected into E. coli cells. HPLC analysis of the modified DNA shows that only dG-C8-AF adducts are formed. Approximately 20 adducts per FRI molecule constitute one lethal event when plaque forming ability is assayed on E. coli cells which have received no prior SOS induction. The mutagenicity of dG-C8-AF adducts was assayed by measuring loss of beta-galactosidase activity as a function of adducts per molecule. A dose-dependent increase in lac(-) mutants was observed, with a four-fold increase in mutants per survivor at 30 adducts per RFI molecule. The mutants were analyzed by DNA sequencing, and occur predominately at either G or C positions which are different from those observed in the spontaneous mutant spectrum. In the assay system, dG-C8-AF adducts induce a previously-unreported recombinogenic activity. (Copyright (c) 1988 Elsevier Science Publishers B.V. (Biomedical Division.))
Supplementary Notes Prepared in cooperation with Texas Univ. System Cancer Center, Smithville.
Availability Notes Pub. in Mutation Research, v201 p203-212 1988.
NTIS Title Notes Journal article.
Title Annotations Reprint: Mutational Spectrum and Recombinogenic Effects Induced by Aminofluorene Adducts in Bacteriophage M13 (Journal Version).
Category Codes 57F; 57K
NTIS Prices Not available NTIS
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 836527127
Origin NTIS
Type CAT