| Main Title |
Mutagenicity Evaluation of Dow Corning 200 Fluid in the Ames Bacterial Assay, with Cover Letter dated 4/20/94. |
| CORP Author |
Dow Corning Corp., Midland, MI.; Environmental Protection Agency, Washington, DC. Office of Toxic Substances. |
| Year Published |
1994 |
| Report Number |
86940001347 |
| Stock Number |
OTS0558128 |
| Additional Subjects |
Toxicology ;
Health effects ;
Dodecamethylpentasiloxane (141-63-9) ;
Genotoxicity ;
Gene Mutations ;
Bacteria ;
In Vitro ;
Toxic substances ;
Laboratory animals ;
CAS No 141-63-9 ;
CAS No 63148-62-9
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
OTS0558128 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
15p |
| Abstract |
This study was conducted to evaluate the mutagenic potential of polydimethylsiloxane (PDMS) fluid (2 and 50 cs.). The test materials were evaluated in Salmonella typhimurium (TA 1535, TA 1537, TA 1538, TA 98, and TA 100) using the Ames assay in the presence and absence of Aroclor induced rat liver enzymes. Dilutions of the test substance were made using deionized water, absolute ethanol, or dimethylsulfoxide (DMSO). The first phase of the study was the spot plate test in which 10 uL of the test substance/plate was spotted directly on cultures containing the microorganisms, with and without activation, and incubated for 48 hours. In the second phase, concentrations of 0 (solvent control), 0.5, 5, 100, or 500 uL of the test substance, or a positive controls, were plated with each tester strain, in presence and absence of microsomal enzymes, and incubated for 48 hours. Sodium azide, 9 amino acridine, 2 nitrofluorene, and 2 anthramine served as positive controls. All the plates were evaluated and compared with positive and negative controls. Under the conditions of this study, the test substances failed to exhibit mutagenic activity both with and without activation. |
| Supplementary Notes |
The quality of the documents listed in the Office of Toxic Substances database may not meet usual NTIS standards but are included to further the opportunity for the scientific and technical community to locate materials which may not be available elsewhere. The content is the responsibility of OTS. Sponsored by Environmental Protection Agency, Washington, DC. Office of Toxic Substances. |
| Availability Notes |
Product reproduced from digital image. Order this product from NTIS by: phone at 1-800-553-NTIS (U.S. customers); (703)605-6000 (other countries); fax at (703)605-6900; and email at orders@ntis.gov. NTIS is located at 5285 Port Royal Road, Springfield, VA, 22161, USA. |
| PUB Date Free Form |
1994 |
| Category Codes |
57Y; 57U; 68G; 99; 44G |
| NTIS Prices |
PC A03/MF A03 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
028803860 |
| Origin |
NTIS |
| Type |
CAT |
