| Main Title |
Analysis of X-ray-Induced HPRT Mutations in CHO Cells: Insertion and Deletions. |
| Author |
Fuscoe, J. C. ;
Zimmerman, L. J. ;
Fekete, A. ;
Setzer, R. W. ;
Rossiter, B. J. F. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Semmelweis Univ. of Medicine, Budapest (Hungary). ;Baylor Coll. of Medicine, Houston, TX. |
| Publisher |
c1992 |
| Year Published |
1992 |
| Report Number |
EPA/600/J-92/437; |
| Stock Number |
PB93-141364 |
| Additional Subjects |
Mutagenesis ;
X-ray irradiation ;
DNA damage ;
Hypoxanthine phosphoribosyltransferase ;
CHO cells ;
DNA mutational analysis ;
Mutations ;
Base sequence ;
Gene expression ;
Polymerase chain reaction ;
Southern blotting ;
Exons ;
Genetic recombination ;
Chromosome deletion ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB93-141364 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
15p |
| Abstract |
Molecular alterations were examined in the hypoxanthine guanine phosphoribosyltransferase (hprt) gene of 41 independent X-ray-induced thioguanine-resistant (TGR) Chinese hamster ovary (CHO) cell clones. Rapid screening of the clones by multiplex polymerase chain reaction (PCR) for the presence or absence of exons revealed that the bases for the mutant phenotype were total gene deletion (26/41), partial gene deletion (4/41), and an insertion (1/41). Southern blot analysis confirmed the deletion data and revealed an additional class of mutants that had a gene disruption but retained all hprt exons (2/41). In addition, the DNA sequence of the insertion mutation was determined. |
| Supplementary Notes |
Pub. in Mutation Research, v269 n2 p171-183 Oct 92. Prepared in cooperation with Environmental Health Research and Testing, Inc., Research Triangle Park, NC., Semmelweis Univ. of Medicine, Budapest (Hungary), and Baylor Coll. of Medicine, Houston, TX. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Analysis of X-ray-Induced HPRT Mutations in CHO Cells: Insertion and Deletions. |
| Category Codes |
57F; 57V |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
307031431 |
| Origin |
NTIS |
| Type |
CAT |
