| Main Title |
Hotspot Sites for Acridine-Induced Frameshift Mutations in Bacteriophage T4 Correspond to Sites of Action of the T4 Type II Topoisomerase. |
| Author |
Ripley, L. S. ;
Dubins, J. S. ;
deBoer, J. G. ;
DeMarini, D. M. ;
Bogerd, A. M. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;New Jersey Medical School, Newark. ;National Inst. of Environmental Health Sciences, Research Triangle Park, NC. ;York Univ., Downsview (Ontario). Dept. of Biology. ;Duke Univ., Durham, NC. |
| Publisher |
c1988 |
| Year Published |
1988 |
| Report Number |
EPA/600/J-88/362; |
| Stock Number |
PB89-237390 |
| Additional Subjects |
Acridines ;
Bacteriophages ;
Mutations ;
Genetics ;
In vivo analysis ;
In vitro analysis ;
Reprints ;
DNA damage ;
Topoisomerase ;
Mutagenicity tests ;
Base sequence
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB89-237390 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
18p |
| Abstract |
The type II topoisomerase of bacteriophage T4 is a central determinant of the frequency and specificity of acridine-induced frameshift mutations. Acridine-induced frameshift mutagenesis is specifically reduced in a mutant defective in topoisomerase activity. The ability of an acridine to promote topoisomerase-dependent cleavage at specific DNA sites in vitro is correlated to its ability to produce frameshift mutations at those sites in vivo. The specific phosphodiester bonds cleaved in vitro are precisely those at which frameshifts are most strongly promoted by acridines in vivo. The cospecificity of in vitro cleavage and in vivo mutation implicate acridine-induced, topoisomerase-mediated DNA cleavages as intermediates of acridine-induced mutagenesis in T4. (Copyright (c) 1988 Academic Press Limited.) |
| Supplementary Notes |
Pub. in Jnl. of Molecular Biology, v200 p665-680 1988. Prepared in cooperation with New Jersey Medical School, Newark, National Inst. of Environmental Health Sciences, Research Triangle Park, NC., York Univ., Downsview (Ontario). Dept. of Biology, and Duke Univ., Durham, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Hotspot Sites for Acridine-Induced Frameshift Mutations in Bacteriophage T4 Correspond to Sites of Action of the T4 Type II Topoisomerase. |
| Category Codes |
57F; 57K |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
931318584 |
| Origin |
NTIS |
| Type |
CAT |
