| Main Title |
Identification and Quantitation of Alkylated Nucleobases by High-Performance Liquid Chromatography with UV Photodiode Array Detection. |
| Author |
Xue, W. ;
Samanta, A. K. ;
Carlson, R. M. ;
|
| CORP Author |
Minnesota Univ.-Duluth. Dept. of Chemistry. ;Academia Sinica, Beijing (China). Research Center for Eco-Environmental Sciences.;Environmental Research Lab.-Duluth, MN. |
| Publisher |
c1990 |
| Year Published |
1990 |
| Report Number |
EPA-R813144-02 ;EPA-R813943; EPA/600/J-90/252; |
| Stock Number |
PB91-144931 |
| Additional Subjects |
Nucleosides ;
Liquid chromatography ;
Ultraviolet detectors ;
Alkylation ;
Molecular structure ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB91-144931 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
7p |
| Abstract |
The application of UV diode array detection in high-performance liquid chromatographic (HPLC) identification and quantification of several classes of synthetic and commercially available alkylated nucleobases is investigated. Quantitative spectral overlays of these compounds to methyl standard references from a spectral library and absorbance ratios at two maximal wavelengths are found to be useful in categorizing the solutes. They can be grouped into classes of compounds originating from a specific nucleobase and classes of analogs having different alkyl substituents (e.g., methyl, ethyl, propyl, allyl, and benzyl) at the same position of the heterocycle. At a selected wavelength for alkylated nucleobases in the same class, the detector response factors are independent of the alkyl group (+ or - 10%). This technique provides a practical means for both qualitative and quantitative analysis of product distribution of DNA base alkylation by using only readily obtainable methylated derivatives as the reference standards. |
| Supplementary Notes |
Prepared in cooperation with Academia Sinica, Beijing (China). Research Center for Eco-Environmental Sciences. Sponsored by Environmental Research Lab.-Duluth, MN. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Identification and Quantitation of Alkylated Nucleobases by High-Performance Liquid Chromatography with UV Photodiode Array Detection. |
| Category Codes |
99A; 57F |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/03 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
109129924 |
| Origin |
NTIS |
| Type |
CAT |
