Main Title |
Cloning, Physical Mapping and Expression of Chromosomal Genes Specifying Degradation of the Herbicide 2,4,5-T by 'Pseudomonas cepacia' AC1100. |
Author |
Sangodkar, U. M. X. ;
Chapman, P. J. ;
Chakrabarty, A. M. ;
|
CORP Author |
Illinois Univ. at the Medical Center, Chicago. Dept. of Microbiology and Immunology.;Environmental Research Lab., Gulf Breeze, FL.;National Inst. of Environmental Health Sciences, Research Triangle Park, NC. |
Publisher |
c1988 |
Year Published |
1988 |
Report Number |
EPA-R-812660 ;PHS-ES-04050-3; EPA/600/J-88/372; |
Stock Number |
PB90-100520 |
Additional Subjects |
Herbicides ;
Pseudomonas ;
Biodeterioration ;
Deoxyribonucleic acids ;
Naphthalene ;
Toulene ;
Reprints ;
Molecular cloning ;
Gene expression regulation ;
Chromosome mapping ;
Pseudomonas cepacia AC1100 ;
T 2-4-5 herbicide ;
Chlorobenzoates ;
Nucleic acid hybridization ;
Nucleic acid sequence homology
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB90-100520 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
13p |
Abstract |
A genomic library of total DNA of Pseudomonas cepacia AC1100 was constructed on a broad-host-range cosmid vector pCP13 in E. coli AC80. A 25-kb segment was isolated from the library that complemented a Tn5-generated, 2,4,5-T minus mutant, P. cepacia PT88. A simple colorimetric assay was developed to detect the presence of this active enzyme in intact cells and was used to determine the expression of complementing genes. Subcloning experiments showed that a 4-kb BamHI-PstI fragment and a 290-bp PstI-EcoRI fragment, separated by 1.3-kb, were required for complementation. Both fragments were identified to be chromosomal in origin. Hybridization studies using the subcloned fragments revealed that in addition to a Tn5 insertion, mutant PT88 contained an extensive chromosomal deletion accounting for its 2,4,5-T-phenotype. The cloned fragments did not show homology to plasmid DNAs carrying degradative genes for toluene, naphthalene, and 3-chlorobenzoate. (Copyright 1988 Elsevier Science Publishers B.V. (Biomedical Division).) |
Supplementary Notes |
Pub. in Gene 71, n2 p267-277 1988. Sponsored in part by grant EPA-R-812011-01. Sponsored by Environmental Research Lab., Gulf Breeze, FL., and National Inst. of Environmental Health Sciences, Research Triangle Park, NC. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Cloning, Physical Mapping and Expression of Chromosomal Genes Specifying Degradation of the Herbicide 2,4,5-T by 'Pseudomonas cepacia' AC1100. |
Category Codes |
57F; 57K; 68E |
NTIS Prices |
PC A03/MF A01 |
Primary Description |
600/04 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
932616377 |
Origin |
NTIS |
Type |
CAT |