Main Title |
Amplification of CCl4 Toxicity by Chlordecone: Destruction of Rat Hepatic Microsomal Cytochrome P-450 Subpopulation. |
Author |
Chaudhury, S. ;
Mehendale, H. M. ;
|
CORP Author |
Mississippi Univ. Medical Center, Jackson. Dept. of Pharmacology and Toxicology.;Health Effects Research Lab., Research Triangle Park, NC.;Harry G. Armstrong Aerospace Medical Research Lab., Wright-Patterson AFB, OH. |
Publisher |
c1991 |
Year Published |
1991 |
Report Number |
EPA-R-814053; EPA/600/J-91/052; |
Stock Number |
PB91-191593 |
Additional Subjects |
Chlordecone ;
Carbon tetrachloride ;
Liver microsomes ;
Cytochrome P-450 ;
Toxicity ;
Liver regeneration ;
Rats ;
Cathechin ;
Ion exchange chromatography ;
Mixed function oxidases ;
Organ weight ;
Liver enzymes ;
Reprints ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB91-191593 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
20p |
Abstract |
Previous work has shown that chlordecone (CD)-amplified CCl4 hepatoxicity and lethality can be mitigated by pretreatment with (+)-cyanidanol. These studies also revealed that stimulated hepatocellular regeneration might play an important role in the cyanidanol protection of CD-amplified CCl4 toxicity. The present studies conducted over a time course of 0 to 120 hr after CCl4 challenge describe sequential changes in hepatic (3)H-thymidine incorporation into hepatocellular nuclear DNA, polyamines and related enzymes, and histomorphometry of liver sections from variously treated rats. Male Sprague-Dawley rats (125-150 g) were maintained on a control diet or on a diet contaminated with CD (10 ppm for 15 days) and/or pretreated with cyanidanol (250 mg/kg, ip) at 48, 24 and 2 hr before a single ip injection of 50 microliter CCl4/kg(L) to 100 microliter CCl4/kg(H) on day 16 of the dietary protocol. Cyanidanol-stimulated (3)H-thymidine incorporation was highly suppressed in rats receiving the CD + CCl4(H) combination treatment up to 36 hr, but after this time point a marked increase was observed. Hepatocellular regeneration, quantified histomorphometrically as volume density of cells in metaphase was progressively increased in rats protected from CD + CCl4 interaction by cyanidanol, starting at 36 hr and lasting until 72 hr. |
Supplementary Notes |
Pub. in Jnl. of Toxicology and Environmental Health, v32 p277-294 Mar 91. Sponsored by Health Effects Research Lab., Research Triangle Park, NC., and Harry G. Armstrong Aerospace Medical Research Lab., Wright-Patterson AFB, OH. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Amplification of CCl4 Toxicity by Chlordecone: Destruction of Rat Hepatic Microsomal Cytochrome P-450 Subpopulation. |
Category Codes |
57Y; 68E |
NTIS Prices |
PC A03/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
119326336 |
Origin |
NTIS |
Type |
CAT |