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Main Title Isolation and Diversity of Actinomycetes in the Chesapeake Bay.
Author Takizawa, M. ; Colwell, R. R. ; Hill, R. T. ;
CORP Author Center of Marine Biotechnology, Baltimore, MD. ;Takeda Chemical Industries Ltd., Osaka (Japan). Discovery Research Labs. II.;Environmental Research Lab., Gulf Breeze, FL.;National Science Foundation, Washington, DC.
Publisher c1993
Year Published 1993
Report Number CONTRIB-196; NSF-BSR9020268; EPA/600/J-93/223;
Stock Number PB93-205060
Additional Subjects Actinomycetales ; Aquatic microbiology ; Chesapeake Bay ; Microbial colony count ; Species diversity ; Nucleic acid hybridization ; Nalidixic acid ; Sediments ; Bacterial DNA ; Reprints ; Actinoplanetes
Library Call Number Additional Info Location Last
NTIS  PB93-205060 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
Abstract Chesapeake Bay was investigated as a source of actinomycetes to screen for production of novel bioactive compounds. The presence of relatively large populations of actinoplanetes (chemotype II/D actinomycetes) in Chesapeake Bay sediment samples indicates that it is an eminently suitable ecosystem from which to isolate actinomycetes for screening programs. Actinomycetes were isolated from sediment samples collected in Chesapeake Bay with an isolation medium containing nalidixic acid, which proved to be more effective than heat pretreatment of samples. Actinomycetes counts ranged from a high of 140,000 to a low of 180 CFU/ml of sediment. Actinomycetes constituted 0.15 to 8.63% of the culturable microbial community. The majority of isolates from the eight stations studied were actinoplanetes (i.e., chemotype II/D), and 249 of these isolates were obtained in a total of 298 actinomycete isolates. Antimicrobial activity profiles indicated that diverse populations of actinoplanetes were present at each station. DNA hybridization studies showed considerable diversity among isolates between stations, but indicated that actinoplanete strains making up populations at nearby stations were more similar to each other than to populations sampled at distant stations. The diversity of actinoplanetes and the ease with which these organisms were isolated from Chesapeake Bay sediments make this a useful source of these actinomycetes. (Copyright (c) 1993, American Society for Microbiology.)
Supplementary Notes Pub. in Applied and Environmental Microbiology, v59 n4 p997-1002 Apr 93. Prepared in cooperation with Takeda Chemical Industries Ltd., Osaka (Japan). Discovery Research Labs. II. Sponsored by Environmental Research Lab., Gulf Breeze, FL., and National Science Foundation, Washington, DC.
NTIS Title Notes Journal article.
Title Annotations Reprint: Isolation and Diversity of Actinomycetes in the Chesapeake Bay.
Category Codes 57K; 47D
NTIS Prices PC A02/MF A01
Primary Description 600/04
Document Type NT
Cataloging Source NTIS/MT
Control Number 321825548
Origin NTIS
Type CAT