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Main Title Influence of Root Colonizing Bacteria on the Defense Responses of Bean.
Author Zdor, R. E. ; Anderson, A. J. ;
CORP Author Utah Agricultural Experiment Station, Logan.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1992
Year Published 1992
Report Number PAPER-4148; EPA/600/J-93/221;
Stock Number PB93-205045
Additional Subjects Pseudomonas ; Bean plants ; Microbial colony count ; Ribonucleic acids ; Nucleic acid hybridization ; Chemiluminescence ; Phenols ; Peroxidases ; Pseudomonas putida ; Reprints ; Plant immunology ; Phaseolus vulgaris ; Pseudomonas tolaasii ; Pseudomonas aureofaciens
Library Call Number Additional Info Location Last
NTIS  PB93-205045 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
Abstract Systemic effects of root inoculation by Pseudomonas putida isolate Corvallis, P. tolaasii (P9A) and P. aureofaciens REW1-I-1 were observed in bean leaves from 14-day-old plants. SDS- polyacrylamide gel electrophoresis demonstrated that levels of certain acid-soluble proteins increased in the leaf extracts of inoculated plants. Plants inoculated with REW1-I-1 produced more of a 57 M(r) protein, and plants inoculated with isolates P9A and REW1-I-1 produced more of a 38 M(r) protein. Northern hybridization revealed enhanced accumulation of mRNAs, that encode the pathogenesis-related protein PR1a, in leaves of plants inoculated with P. putida and REW1-I-1. Only REW1-I-1, but not P9A or P. putida induced symptoms of an hypersensitive response on tobacco leaves, bean cotyledons, and in bean suspension cultures. Phenolics and phytoalexins accumulated in bean cotyledons exposed to REW1-I-1 for 24h but little change in levels of these compounds occurred in cotyledons inoculated with P9A and P. putida. Both suspension culture cells and roots treated with REW1-I-1 rapidly evolved more hydrogen peroxide than those exposed to P9A and P. putida. However, roots from 14-day-old plants colonized by P9A, P. putida or REW1-I-1 did not have higher levels of phenolics, phytoalexins or mRNAs for two enzymes involved in phenolic biosynthesis, phenylalanine-ammonia lyase and chalcone synthase. A selective induction of plant defense strategies upon root colonization by certain pseudomonads is apparent. (Copyright (c) 1992 Kluwer Academic Publishers.)
Supplementary Notes Pub. in Plant and Soil 140, p99-107 1992. Sponsored by Environmental Research Lab., Gulf Breeze, FL.
Title Annotations Reprint: Influence of Root Colonizing Bacteria on the Defense Responses of Bean.
Category Codes 57K; 57C; 98D
NTIS Prices PC A03/MF A01
Document Type NT
Cataloging Source NTIS/MT
Control Number 323828417
Origin NTIS
Type CAT