Main Title |
Mouse ear Swelling Test (Sensitization Assay) of Di-n-butylamine. |
CORP Author |
Bio-Dynamics Research and Development Corp., Eugene, OR.; Environmental Protection Agency, Washington, DC. Office of Toxic Substances. |
Year Published |
1987 |
Report Number |
86-870000820 |
Stock Number |
OTS0515258 |
Additional Subjects |
Toxicology ;
Health effects ;
Di(n-butyl)amine (111-92-2) ;
Primary Dermal Sensitization ;
Mammals ;
Mice ;
Dermal ;
Toxic substances ;
Laboratory animals ;
CAS No 111-92-2
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Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
OTS0515258 |
Some EPA libraries have a fiche copy filed under the call number shown. |
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07/26/2022 |
|
Collation |
20p |
Abstract |
The dermal sensitization potential of di-n-butylamine (CAS No. 111-92-2) was evaluated in 10 CF1(BR) mice inducted with 3 daily 0.1 mL topical applications of a 0.1% (v/v in ethanol) test solution to clipped abdomens. A group of 10 mice likewise inducted over 3 days with 0.1 mL dermal applications of 0.5% (w/v) DNCB served as the positive control. Challenge and rechallenge were administered 7 and 14 days later in .01 mL dermal applications of a 25% (v/v) solution to both dorsal and ventral surfaces of the left and right ears, respectively, of inducted test mice. The thickness of treated left ears relative to that of solvent control (ethanol) right ears indicated the degree of sensitization. One animal challenged with di-n-butylamine exhibited a positive sensitization response (20% increase in ear thickness over control) at 48-hour evaluation only. None of either test or irritation control groups had responded to challenge by the 24-hour evaluation and none responded to rechallenge. Conversely, 60% and 50% positive response in the DCNB-inducted and challenged groups at 24-hour and 48-hour evaluations, respectively, confirmed the validity of the test system. |
Supplementary Notes |
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Availability Notes |
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PUB Date Free Form |
1987 |
Category Codes |
57Y; 57U; 68G; 99; 44G |
NTIS Prices |
PC A03/MF A03 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
028802020 |
Origin |
NTIS |
Type |
CAT |