| Main Title |
Immunohistochemical Detection of Tumour-Associated Aldehyde Dehydrogenase in Formalin-Fixed Rat and Mouse Normal Liver and Hepatomas. |
| Author |
Richmond, R. E. ;
DeAngelo, A. B. ;
Daniel, F. B. ;
Lindahl, R. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;Northern Kentucky Univ., Highland Heights. Dept. of Biological Sciences. ;Alabama Univ., University. |
| Publisher |
c1990 |
| Year Published |
1990 |
| Report Number |
EPA/600/J-90/355; |
| Stock Number |
PB91-163691 |
| Additional Subjects |
Aldehyde dehydrogenase ;
Hepatoma ;
Liver ;
Rats ;
Mice ;
Immunohistochemistry ;
Formalin ;
Fixatives ;
Carcinogenicity tests ;
Carcinogens ;
Dichloroacetate ;
Diethylnitrosamine ;
Ethylnitrosourea ;
Tumor markers ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB91-163691 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
6p |
| Abstract |
The paper describes a method and the results of the immunohistochemical (IHC) detection of tumor-associated isozyme of aldehyde dehydrogenase (B-ALDH). The method can be used to demonstrate B-ALDH expression in cells of formalin-fixed, low melting-point paraffin-embedded liver tissue of rats and mice. Thus the method is a substantial improvement over standard histochemical detection methods which require either frozen, or mild preservative-fixed tissue sections. Using the IHC method, B-ALDH expression was detected in hepatocarcinomas of Sprague-Dawley rats treated with the carcinogen diethylnitrosamine (DENA) and in mice treated with either DENA, ethylnitrosourea (ENU), or dichloroacetic acid (DCA). B-ALDH expression has not been previously reported in mice treated with the latter two carcinogens and these results substantiate further the use of B-ALDH as a histochemical marker for mouse hepatocarcinogenesis. |
| Supplementary Notes |
Pub. in Histochemical Jnl., v22 n10 p526-529 Oct 90. Prepared in cooperation with Northern Kentucky Univ., Highland Heights. Dept. of Biological Sciences, and Alabama Univ., University. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Immunohistochemical Detection of Tumour-Associated Aldehyde Dehydrogenase in Formalin-Fixed Rat and Mouse Normal Liver and Hepatomas. |
| Category Codes |
57F; 57O |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
113027518 |
| Origin |
NTIS |
| Type |
CAT |
