| Main Title |
Guinea Pig Maximization Test: Rabbit Alveolar Macrophage (RAM) Test; and Cell Transformation Test with Cover Letter dated 10/02/1986. |
| CORP Author |
American Cyanamid Co., Wayne, NJ.;Environmental Protection Agency, Washington, DC. Office of Toxic Substances. |
| Publisher |
6 Oct 1986 |
| Year Published |
1986 |
| Report Number |
FYI-OTS-1086-0516; |
| Stock Number |
OTS-0000516-0 |
| Additional Subjects |
Morphological transformation ;
Embryo cells ;
Sampling ;
Quality assurance monitors ;
Procedures ;
Models ;
Documentation ;
Equipment ;
Mice ;
Laboratory animals ;
Nickel containing catalysts ;
Cell culture transformation systems ;
Exogenous metabolic activation ;
Mouse embryo cells ;
BALB/3T3
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
OTS-0000516-0 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
83p |
| Abstract |
Cell transformation represents a number of phenotypic changes observed in carcinogen-treated cells, including morphological alterations, anchorage independence and the acquisition of tumorigenicity on inoculation into suitable rodent hosts. Several cell culture transformation systems have been reviewed and reported to represent valid models for in vivo carcinogenesis . The BAIB/3T3 transformation assay was designed to allow the expression of transformed foci of high cell density and aberrant cell morphology an a confluent monolayer of nontransformed, contact-inhibited 3T3 cells. This system bas been demonstrated to be sensitive to the transforming activity of a variety of carcinogens. The BAIB/3T3 Clone A31-1 cell line was derived by Dr. T. Yakunaga, National Cancer Institute, Bethesda, MD from the aneuploid, nontumorigenic BAIB/3T3 Clone A31 cell line . This subclone is characterized by post-influence inhibition of proliferation and the ability to form distinct quantifiable foci of aberrant cell morphology following exposure to chemical carcinogens. The purpose of this study is to assess the morphological transforming potential of a series of structurally related test articles using the BAIB/3T3 done A31-1 mouse embryo cells in the absence of an exogenous metabolic activation system. |
| Supplementary Notes |
The quality of the documents listed in the Office of Toxic Substances database may not meet usual NTIS standards but are included to further the opportunity for the scientific and technical community to locate materials which may not be available elsewhere. The content is the responsibility of OTS. Prepared in cooperation with American Petroleum Inst., Washington, DC. |
| Availability Notes |
Product reproduced from digital image. Order this product from NTIS by: phone at 1-800-553-NTIS (U.S. customers); (703)605-6000 (other countries); fax at (703)605-6900; and email at orders@ntis.gov. NTIS is located at 5285 Port Royal Road, Springfield, VA, 22161, USA. |
| Category Codes |
57F |
| NTIS Prices |
PC A06/MF$25.00 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
019100960 |
| Origin |
NTIS |
| Type |
CAT |
