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RECORD NUMBER: 201 OF 1249

Main Title Distribution of Urethane and Its Binding to DNA, RNA, and Protein in SENCAR and BALB/c Mice Following Oral and Dermal Administration.
Author Fossa, A. A. ; Baird, W. M. ; Carlson, G. P. ;
CORP Author Purdue Univ., Lafayette, IN.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1985
Report Number EPA-R-809744; EPA/600/J-85/401;
Stock Number PB86-200862
Additional Subjects Urethane ; Toxicology ; Deoxyribonucleic acids ; Ribonucleic acids ; Binding ; Mice ; Laboratory animals ; Proteins ; Metabolism ; Reprints ; Tumorigenesis
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NTIS  PB86-200862 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 22p
Abstract In the initiation-promotion (I/P) assay for mouse skin tumorigenesis the initiating activity of various compounds is dependent upon both route of administration and strain of mouse tested. Urethane produces 3-fold more skin papillomas per mouse when administered orally than dermally in Sencar mice. To examine the biochemical basis for route and strain differences in the I/P assay, the binding of 14-C-urethane to DNA, RNA, and protein in mice susceptible (Sencar) and relatively resistant (Balb/c) to tumorigenesis by the protocol was determined. Binding of 14-C-urethane (0.062 mg/g body weight, 50 uCi/20 g body weight) to DNA, RNA and protein 6 hours after oral administration varied with tissue (liver > stomach > skin = lung) but not with strain. Binding to DNA in skin, lung and stomach, RNA in stomach and protein in stomach and liver after 48 hours was significantly higher in Sencar than in Balb/c. Dermal application of 14-C-urethane resulted in several fold higher binding to liver DNA of Sencar mice than Balb/c mice. Forty-eight hours after dermal application, significantly higher levels of 14-C-urethane remained bound to skin DNA, RNA and protein Balb/c mice, although all values were lower than at 6 hours after treatment.
Supplementary Notes Pub. in Jnl. of Toxicology and Environmental Health 15, p635-654 May 85. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
NTIS Title Notes Journal article.
Title Annotations Reprint: Distribution of Urethane and Its Binding to DNA, RNA, and Protein in SENCAR and BALB/c Mice Following Oral and Dermal Administration.
PUB Date Free Form c1985
Category Codes 6T; 6E; 57Y; 57O
NTIS Prices PC A02/MF A01
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 621322674
Origin NTIS
Type CAT