| Main Title |
Control of Alkaline Phosphatase Activity in C3H10T1/2 Cells: Role of Retinoic Acid and Cell Density. |
| Author |
Reese, D. H. ;
Larsen, R. A. ;
Hornicek, F. J. ;
|
| CORP Author |
Environmental Protection Agency, Washington, DC. Office of Health and Environmental Assessment. ;AScI Corp., McLean, VA. |
| Publisher |
cSep 91 |
| Year Published |
1991 |
| Report Number |
EPA/600/J-92/239 ;OHEA-R-447; |
| Stock Number |
PB92-198639 |
| Additional Subjects |
Tretinoin ;
Alkaline phosphatase ;
Cells(Biology) ;
Mice ;
Neoplasms ;
Serum-free media ;
Up-regulation(Physiology) ;
Enzyme induction ;
Dose-response relationships ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB92-198639 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
12p |
| Abstract |
The authors have begun to use the chemically-transformable mouse embryo fibroblast cell, C3H10T1/2, to investigate that altered AP expression in some cancers may be caused by a defect in the ability of the cells to respond normally to retinoid. In the initial study they characterized AP regulation in normal C3H10T1/2 cells and show that: (1) RA increases AP activity within 3-4 h in serum-free medium; (2) serum inhibits short-term induction (0-8 h) in a concentration-dependent manner (10% serum causes complete inhibition); (3) during long-term RA exposure (24 h and 48 h), induction can be detected in serum-containing medium; (d) AP induction is dose related in serum-free medium; (5) ten to the minus fifth power M RA is ineffective at inducing AP in serum-free medium during 8 h but is the most effective concentration in serum-containing medium during 24 h and 48 h exposures; (6) AP inducibility by RA requires near-confluent cell densities; and, (7) when cultures become confluent, cells become constitutive for AP and no longer require RA for enzyme expression. |
| Supplementary Notes |
Pub. in Jnl. of Cellular Physiology, v151 p239-248 1992. Prepared in cooperation with AScI Corp., McLean, VA. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Control of Alkaline Phosphatase Activity in C3H10T1/2 Cells: Role of Retinoic Acid and Cell Density. |
| Category Codes |
57F; 57B; 57Q |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/21 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
224126826 |
| Origin |
NTIS |
| Type |
CAT |
