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Main Title 'Spodoptera frugiperda' Nuclear Polyhedrosis Virus Genome: Physical Maps for Restriction Endonucleases BamHI and HindIII.
Author Loh, Lambert C. ; Hamm, John J. ; Huang, Eng-Shang ;
CORP Author North Carolina Univ. at Chapel Hill.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1981
Report Number EPA-R-806210; EPA-600/J-81-517;
Stock Number PB82-153768
Additional Subjects Genes ; Viruses ; Maps ; Microorganisms ; Genetics ; Reprints ; Spodoptera frugiperda ; Nuclear polyhedrosis viruses ; Endonucleases
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NTIS  PB82-153768 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
Abstract The physical map for the genome of Spodoptera frugiperda nuclear polyhedrosis virus was constructed for restriction endonucleases BamHI and HindIII. The ordering of the restriction fragments was accomplished by cross-blot hybridization of BamHI, HindIII, and EcoRI fragments. The alignment of the HindIII fragments within the BamHI map was achieved by double digestion with the two restriction endonucleases followed by cross-blot hybridization. The results showed that the viral genome consisted of mainly unique sequences. In addition, the circular nature of the viral genome was reaffirmed.
Supplementary Notes Pub. in Jnl. of Virology, v38 n3 p922-931 Jun 81. Prepared in cooperation with Department of Agriculture, Tifton, GA.
NTIS Title Notes Journal article.
Title Annotations Reprint: 'Spodoptera frugiperda' Nuclear Polyhedrosis Virus Genome: Physical Maps for Restriction Endonucleases BamHI and HindIII.
PUB Date Free Form 9 Mar 81
Category Codes 6M; 6C; 57K; 57F
NTIS Prices PC A02/MF A01
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 323810202
Origin NTIS
Type CAT