| Main Title |
Heterogeneity of Human Natural Killer (NK) Cells: Enrichment of NK by Negative-Selection with the Lectin from 'Erythrina cristagalli' (Journal Version). |
| Author |
Harris, D. T. ;
Iglesias, J. L. ;
Argov, S. ;
Toomey, J. ;
Koren, H. S. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;North Carolina Univ. at Chapel Hill. ;Duke Univ. Medical Center, Durham, NC. ;Ben-Gurion Univ. of the Negev, Beersheba (Israel). |
| Publisher |
c1987 |
| Year Published |
1987 |
| Report Number |
EPA/600/J-87/431; |
| Stock Number |
PB89-105878 |
| Additional Subjects |
Immunology ;
Lectins ;
Natural killer cells ;
Antibody-dependent cell cytotoxicity ;
Humans ;
Lymphokines ;
Isolation ;
Lymphocytes ;
Reprints ;
Erythrina cristagalli
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB89-105878 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
9p |
| Abstract |
A novel technique for the isolation and enrichment of human natural killer (NK) cells from peripheral blood mononuclear cells (MNC) is described. Negative selection of MNC with the lectin from Erythrina cristagalli (ECA), whether by panning or agglutination in solution, resulted in a population of lymphocytes (5-20% of original MNC) highly enriched in cells exhibiting NK function. The enrichment was evident by a significant increase (range of 3-50-fold) in cells with large granular lymphocyte (LGL) morphology, K562 tumor-binding cells, cytotoxic activity, and cells expressing NK phenotypic markers (Leu 11+, OKM1+). Analysis of the cytolytic specificity of the cells demonstrated that the lytic spectrum was typical of endogenous NK. The effector cells were responsive to augmentation of cytotoxic potential by lymphokines (IL-2, IFN-alpha, and IFN-gamma) and capable of antibody-dependent cell-mediated cytotoxicity (ADCC). ECA-negative (ECA(-)) cells were equivalent to NK isolated by Percoll gradient fractionation. NK heterogeneity was demonstrated by the observation of a small percentage (1-5% of MNC) of NK in the ECA(+) population. (Copyright (c) 1987 Alan R. Liss, Inc.) |
| Supplementary Notes |
Pub. in Jnl. of Leukocyte Biology, v42 p163-170 1987. Prepared in cooperation with North Carolina Univ. at Chapel Hill, Duke Univ. Medical Center, Durham, NC., and Ben-Gurion Univ. of the Negev, Beersheba (Israel). |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Heterogeneity of Human Natural Killer (NK) Cells: Enrichment of NK by Negative-Selection with the Lectin from 'Erythrina cristagalli' (Journal Version). |
| Category Codes |
57J |
| NTIS Prices |
Not available NTIS |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
833027566 |
| Origin |
NTIS |
| Type |
CAT |
