| Main Title |
Direct DNA Sequencing of PCR Products. |
| Author |
Zimmerman, L. J. ;
Fuscoe, J. C. ;
|
| CORP Author |
Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. |
| Publisher |
c1991 |
| Year Published |
1991 |
| Report Number |
EPA/600/J-92/053; |
| Stock Number |
PB92-150671 |
| Additional Subjects |
Deoxyribonucleic acids ;
Polymerase chain reaction ;
Hypoxanthine phosphoribosyltransferase ;
Polyacrylamide gel electrophoresis ;
Southern blotting ;
T-lymphocytes ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB92-150671 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
5p |
| Abstract |
Experiments are described which elucidate some of the technical problems associated with the direct sequencing of polymerase chain reaction (PCR) amplified DNA. Sequencing primer purity, labeling methodology, and template preparation were explored. Conditions are presented for the routine sequencing of single- and double-stranded PCR products. (Copyright (c) 1991 Wiley-Liss, Inc.) |
| Supplementary Notes |
Pub. in Environmental and Molecular Mutagenesis, v18 n4 p274-276 Nov 91. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Direct DNA Sequencing of PCR Products. |
| Category Codes |
57F |
| NTIS Prices |
PC A01/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
211522805 |
| Origin |
NTIS |
| Type |
CAT |
