Full Record Display for the EPA National Library Catalog

RECORD NUMBER: 14 OF 23

Main Title Molecular Dissection of Mutations at the Heterozygous Thymidine Kinase Locus in Mouse Lymphoma Cells.
Author Applegate, M. L. ; Moore, M. M. ; Broder, C. B. ; Burrell, A. ; Hozier, J. C. ;
CORP Author Florida State Univ., Tallahassee. Dept. of Biological Science. ;Florida Inst. of Tech., Melbourne. Dept. of Biological Science. ;Yale Univ., New Haven, CT. Dept. of Biology. ;IBM Research Div., San Jose, CA.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/040;
Stock Number PB90-217548
Additional Subjects Mutations ; Lymphoma ; Mutagens ; Mice ; Reprints ; Thymidine kinase ; Chromosome mapping ; Restriction fragment length polymorphism ; Cultured tumor cells ; Southern immunoblotting
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB90-217548 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
Abstract The mouse lymphoma L5178Y TK+/- 3.7.2C cell line allows quantitation of induced TK +/- -> TK-/- mutations at the heterozygous thymidine kinase (tk) locus. TK-/- mutant colonies show a bimodal size distribution, reflecting a difference in the growth rates of the two size classes that is hypothesized to result from different degrees of genetic damage. The two homologous chromosomes containing the alleles of the tk gene in L5178Y 3.7.2C TK+/- cells are distinguishable at the cytogenetic level by the unique centromeric morphology of each of the two chromosomes 11, to which the tk gene has been mapped in mouse cells. In addition, the two alleles were distinguishable at the molecular level because of an Nco I restriction-fragment-length polymorphism at the 3' end of the gene. In a set of 51 large-colony and 48 small-colony TK-/- mutants induced by ionizing radiation or by chemical mutagens, 78, including all except one of the small-colony mutants, have lost the tk+ allele and that some of these have two to four copies of the remaining, tk-, allele. Nineteen of the large-colony TK-/- mutants that do not show tk+ allele loss show no other structural changes detectable at the level of Southern blot analysis.
Supplementary Notes Pub. in Proceedings of the National Academy of Sciences USA, v87 p51-55 Jan 90. Prepared in cooperation with Florida Inst. of Tech., Melbourne. Dept. of Biological Science, Yale Univ., New Haven, CT. Dept. of Biology, and IBM Research Div., San Jose, CA. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
NTIS Title Notes Journal article.
Title Annotations Reprint: Molecular Dissection of Mutations at the Heterozygous Thymidine Kinase Locus in Mouse Lymphoma Cells.
Category Codes 57F
NTIS Prices PC A02/MF A01
Primary Description 600/10
Document Type NT
Cataloging Source NTIS/MT
Control Number 019411227
Origin NTIS
Type CAT