Main Title |
Effect of Repeated Organophosphate Administration on Carbachol-Stimulated Phosphoinositide Hydrolysis in the Rat Brain. |
Author |
Mundy, W. R. ;
Ward, T. R. ;
Dulchinos, V. F. ;
Tilson, H. A. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div. |
Publisher |
c1993 |
Year Published |
1993 |
Report Number |
EPA/600/J-93/374; |
Stock Number |
PB93-229003 |
Additional Subjects |
Organophosphorus compounds ;
Carbachol ;
Phosphoinositides ;
Isoflurophate ;
Hydrolysis ;
Inositol phosphates ;
Muscarinic receptors ;
Hippocampus ;
Second messenger system ;
Disulfoton ;
Myoinositol ;
Lithium ;
Dose-response relationships ;
Reprints ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB93-229003 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
8p |
Abstract |
The effects of repeated exposure to two organophosphates on the turnover of phosphoinositides, the second messenger system coupled to the M1 and M3 subtypes of muscarinic receptors, were examined in the rat hippocampus. Repeated diisopropylfluorophosphate (DFP) exposure (0.2-0.8 mg/kg, SC) decreased brain acetylcholinesterase activity and muscarinic receptor density. The incorporation of (3H)myoinositol into brain slices was also decreased. Phosphoinositide turnover was measured as the accumulation of (3H)inositol phosphates (IP) in the presence of lithium. DFP did not affect basal IP accumulation, but decreased carbachol-stimulated IP accumulation in the hippocampus after 0.4 and 0.8 mg/kg. The effects of repeated disulfoton administration (2.0 mg/kg, IP) were also examined in the hippocampus. Similar to DFP, repeated disulfoton exposure decreased acetylcholinesterase activity, receptor density, and carbachol-stimulated IP accumulation. The incorporation of myoinositol, however, was increased in disulfoton-treated rats. These data indicate that repeated organophosphate exposure results in a functional decrease in muscarinic receptor activity, as well as changes in myoinositol incorporation into phospholipids. |
Supplementary Notes |
Pub. in Pharmacology Biochemistry and Behavior, v45 n2 p309-314 Jun 93. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Effect of Repeated Organophosphate Administration on Carbachol-Stimulated Phosphoinositide Hydrolysis in the Rat Brain. |
Category Codes |
57Y; 57B; 57Q; 68E |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
329432500 |
Origin |
NTIS |
Type |
CAT |