||Glucocorticoid Receptor Expression during the Development of the Embryonic Mouse Secondary Palate.
Abbott, B. D. ;
McNabb, F. M. A. ;
Lau, C. ;
||Health Effects Research Lab., Research Triangle Park, NC. Developmental Toxicology Div. ;Virginia Polytechnic Inst. and State Univ., Blacksburg. Dept. of Biology.
Glucocorticoid receptors ;
Cleft palate ;
Embryo development ;
Animal pregnancy ;
Messenger RNA ;
In situ hybridization ;
Developmental gene expression regulation ;
Northern blotting ;
Cell differentiation ;
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||Glucocorticoids are known to be teratogenic, and administration of these compounds during pregnancy produces cleft palate in the offspring. In the mouse, induction of cleft palate correlates with the level of palatal glucocorticoid receptor (GR) expression. In the present study, GR expression was evaluated in the developing palates of C57BL/6N mouse embryos on gestation days (GD) 12, 13, 14, and 15 by both in situ hybridization and immunohistochemistry. Chondrogenic regions strongly expressed GR. In different regions of oral and nasal epithelia localized patterns of expression were noted and may be related to differentiated state. Correspondingly, GR mRNA was expressed in epithelia and became regional in mesenchyme with abundant mRNA in regions of bone formation. GR localized initially within regions of active morphogenesis and subsequently within differentiating cells. This specificity of spatial and temporal expression supports the idea that GR is involved in regulation of regional growth and differentiation during palatogenesis.
||Pub. in Jnl. of Craniofacial Genetics and Developmental Biology, v14 p87-96 May 94. Prepared in cooperation with Virginia Polytechnic Inst. and State Univ., Blacksburg. Dept. of Biology.
|NTIS Title Notes
||Reprint: Glucocorticoid Receptor Expression during the Development of the Embryonic Mouse Secondary Palate.
||PC A03/MF A01