Main Title |
Determination of 2,3,7,8-TCDD Toxic Equivalent Factors (TEFs): Support for the Use of the In vitro AHH Induction Assay. |
Author |
Safe, S. ;
|
CORP Author |
Texas A and M Univ., College Station.;Environmental Research Lab.-Duluth, MN. |
Publisher |
c1987 |
Year Published |
1987 |
Report Number |
EPA-810995; EPA/600/J-87/527; |
Stock Number |
PB90-264987 |
Additional Subjects |
Toxicity ;
In vitro analysis ;
Bioassay ;
Reprints ;
Tetrachlorodibenzodioxin ;
Enzyme induction ;
Aryl hydrocarbon hydroxylase ;
Cultured tumor cells ;
Hepatoma
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB90-264987 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
13p |
Abstract |
The in vitro induction of the cytochrome P1-450-dependent monooxygenases, aryl hydrocarbon hydroxylase (AHH) or ethoxyresorufin O-deethylase (EROD) by 2,3,7,8-TCDD and related toxic halogenated aryl hydrocarbons in rat hepatoma H-4-II E cells has been developed as a short term quantitative bioassay for these toxic chemicals. There was a linear correlation between the -log EC50 (in vitro) AHH induction vs the -log ED50 (in vivo) for body weight loss, thymic atrophy, hepatic AHH and EROD induction in the rat for several polychlorinated biphenyl, dibenzo-p-dioxin and dibenzofuran congeners and mixtures. These data clearly support the utility of the in vitro AHH induction assay as a short term test system for quantitating the 'toxic or 2,3,7,8-TCDD equivalent' in an extract containing toxic halogenated aromatics. The bioassay method is rapid, relatively accurate and much more cost effective than conventional analytical methods such as gas chromatography-mass spectrometry from which it is difficult to determine the levels of 2,3,7,8-TCDD equivalents in specific analytes. |
Supplementary Notes |
Pub. in Chemosphere, v16 n4 p791-802 1987. Sponsored by Environmental Research Lab.-Duluth, MN. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Determination of 2,3,7,8-TCDD Toxic Equivalent Factors (TEFs): Support for the Use of the In vitro AHH Induction Assay. |
Category Codes |
57Y; 57F |
NTIS Prices |
PC A03/MF A01 |
Primary Description |
600/03 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
028533221 |
Origin |
NTIS |
Type |
CAT |