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Main Title Determination of 2,3,7,8-TCDD Toxic Equivalent Factors (TEFs): Support for the Use of the In vitro AHH Induction Assay.
Author Safe, S. ;
CORP Author Texas A and M Univ., College Station.;Environmental Research Lab.-Duluth, MN.
Publisher c1987
Year Published 1987
Report Number EPA-810995; EPA/600/J-87/527;
Stock Number PB90-264987
Additional Subjects Toxicity ; In vitro analysis ; Bioassay ; Reprints ; Tetrachlorodibenzodioxin ; Enzyme induction ; Aryl hydrocarbon hydroxylase ; Cultured tumor cells ; Hepatoma
Library Call Number Additional Info Location Last
NTIS  PB90-264987 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13p
Abstract The in vitro induction of the cytochrome P1-450-dependent monooxygenases, aryl hydrocarbon hydroxylase (AHH) or ethoxyresorufin O-deethylase (EROD) by 2,3,7,8-TCDD and related toxic halogenated aryl hydrocarbons in rat hepatoma H-4-II E cells has been developed as a short term quantitative bioassay for these toxic chemicals. There was a linear correlation between the -log EC50 (in vitro) AHH induction vs the -log ED50 (in vivo) for body weight loss, thymic atrophy, hepatic AHH and EROD induction in the rat for several polychlorinated biphenyl, dibenzo-p-dioxin and dibenzofuran congeners and mixtures. These data clearly support the utility of the in vitro AHH induction assay as a short term test system for quantitating the 'toxic or 2,3,7,8-TCDD equivalent' in an extract containing toxic halogenated aromatics. The bioassay method is rapid, relatively accurate and much more cost effective than conventional analytical methods such as gas chromatography-mass spectrometry from which it is difficult to determine the levels of 2,3,7,8-TCDD equivalents in specific analytes.
Supplementary Notes Pub. in Chemosphere, v16 n4 p791-802 1987. Sponsored by Environmental Research Lab.-Duluth, MN.
NTIS Title Notes Journal article.
Title Annotations Reprint: Determination of 2,3,7,8-TCDD Toxic Equivalent Factors (TEFs): Support for the Use of the In vitro AHH Induction Assay.
Category Codes 57Y; 57F
NTIS Prices PC A03/MF A01
Primary Description 600/03
Document Type NT
Cataloging Source NTIS/MT
Control Number 028533221
Origin NTIS
Type CAT