||Comparative Effects of Two Polychlorinated Biphenyl Congeners on Calcium Homeostasis in Rat Cerebellar Granule Cells.
Kodavanti, P. R. S. ;
Shin, D. S. ;
Tilson, H. A. ;
Harry, G. J. ;
||National Inst. of Environmental Health Sciences, Research Triangle Park, NC. Div. of Intramural Research.;Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div.
Polychlorobiphenyl compounds ;
In vitro analysis ;
Ca(2+)-transporting ATPase ;
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||We compared the effects of two polychlorinated biphenyls (PCB) congeners on Ca(2+) homeostasis in cerebellar granule cells: 2,2'-dichlorobiphenyl (DCBP), a putative neurotoxic congener, and 3,3',4,4',5-pentachlorobiphenyl (PCBP), a presumed nonneurotoxic congener. In cerebellar granule cells (6-8 days in vitro), DCBP was cytotoxic as indicated by a significant increase in LDH leakage at 200 microMols after 2 hr of exposure and at 100 microMols after 4 hr exposure. PCBP, on the other hand, did not affect LDH leakage even at 200 microMols for up to 4 hr. Although both congeners increased cerebellar granule cell (Ca(2+))i, DCPB was more effective in increasing (Ca(2+))i to a greater extent than PCBP. The increase in (Ca(2+))i produced by both congeners was not transient, but a steady rise was observed with time. The results of this study support the hypothesis that the position of chlorine substitution on the biphenyl ring and/or number of chlorine substitutions may have significant implications for predicting potential effects of PCB congeners in the nervous system, and perturbations in Ca(2+) homeostasis might play a significant role in the neuroactivity of PCBs.
||Pub. in Toxicology and Applied Pharmacology 123, n1 p97-106 Nov 93. Color illustrations reproduced in black and white. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div.
|NTIS Title Notes
||Reprint: Comparative Effects of Two Polychlorinated Biphenyl Congeners on Calcium Homeostasis in Rat Cerebellar Granule Cells.
||57Y; 57S; 57F
||PC A03/MF A01