| Main Title |
Spatial Distribution of Sperm-Derived Chromatin in Zygotes Determined by Fluorescence In situ Hybridization. |
| Author |
Brandriff, B. F. ;
Gordon, L. A. ;
|
| CORP Author |
Lawrence Livermore National Lab., CA. Biomedical Sciences Div.;Environmental Protection Agency, Washington, DC. Office of Health and Environmental Assessment.;Department of Energy, Washington, DC. |
| Publisher |
c1992 |
| Year Published |
1992 |
| Report Number |
DE-W-7405-ENG-48; EPA/600/J-93/172 ; OHEA-R-475 |
| Stock Number |
PB93-194405 |
| Additional Subjects |
Spermatozoa ;
Chromatin ;
Zygote ;
Fluorescence in situ hybridization ;
Humans ;
DNA probes ;
Hamsters ;
Human pair 19 chromosomes ;
Y chromosome ;
Heterochromatin ;
Human pair 4 chromosomes ;
Reprints ;
Female genetic risk
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB93-194405 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
12p |
| Abstract |
Fluorescence in situ hybridization was used to determine the spatial distribution of chromatin in zygote pronuclei. A hybrid system involving golden hamster eggs and individual human sperm permitted use of DNA probes specific for the entire human chromosome 4, for the heterochromatic region on the long arm of the human Y chromosome and for unique DNA sequences on human chromosome 19. Chromosome 4 occupied a circumscribed domain in the pronuclei, similar to findings in somatic interphases. Unlike the situation in somatic interphases, the Y heterochromatin was extended throughout the first cell cycle. Pronuclear chromatin was extended 3- to 4-fold compared to somatic interphase chromatin. The extended pronuclear chromatin conformation is likely to affect a zygote's susceptibility to environmental hazards. (Copyright (c) 1992 Elsevier Science Publishers B.V.) |
| Supplementary Notes |
Pub. in Mutation Research Special Issue, 'Female Germ Cells: Biology and Genetic Risk', v296 n1/2 p33-42 Dec 92. Color illustrations reproduced in black and white. Sponsored by Environmental Protection Agency, Washington, DC. Office of Health and Environmental Assessment, and Department of Energy, Washington, DC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Spatial Distribution of Sperm-Derived Chromatin in Zygotes Determined by Fluorescence In situ Hybridization. |
| Category Codes |
57F |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/21 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
318931929 |
| Origin |
NTIS |
| Type |
CAT |
