||Effects of Depletion of Ascorbic Acid or Nonprotein Sulfhydryls on the Acute Inhalation Toxicity of Nitrogen Dioxide, Ozone, and Phosgene.
Slade, R. ;
Highfill, J. W. ;
Hatch, G. E. ;
||Health Effects Research Lab., Research Triangle Park, NC.
Nitrogen oxide ;
Ascorbic acid ;
Guinea pigs ;
Pulmonary edema ;
Bronchoalveolar lavage fluid ;
Pulmonary function tests
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||The effect of depleting lung ascorbic acid (AH(sub 2)) and nonprotein sulfhydryls (NPSH) on the acute inhalation toxicity of nitrogen dioxide (NO(sub 2)), ozone (O(sub 3)), and phosgene (COCl(sub 2)) was investigated in guinea pigs. The increase in bronchoalveolar lavage (BAL) fluid protein (an indicator of alveolar-capillary damage leading to increased permeability) was measured 16 to 18 hr following a 4 hr exposure to the gas in animals deficient in (AH(sub 2)) or NPSH. Gas concentrations were chosen which produced low but significant increases in BAL protein. Lung (AH(sub 2)) was lowered to about 20% of control by feeding rabbit chow for 2 weeks. Lung NPSH was lowered to about 50% of control by injecting a mixture of buthionine S,R-sulfoximine (BSO) and diethylmaleate (DEM) (2.7 and 1.2 mmol/kg respectively). BSO/DEM did not affect the lung concentrations of (AH(sub 2)) or alpha-tocopherol. AH(sub 2) depletion caused a 6 fold and a 3 fold enhancement in the toxicity of 5 ppm and 10 ppm (NO(sub 2)), and a 6 fold enhancement in the toxicity of 0.5 ppm (O(sub 3)), but did not affect toxicity of 1.0 ppm (O(sub 3)). AH(sub 2) depletion did not affect phosgene toxicity (at 0.25 ppm and 0.5 ppm). (Copyright (c) 1989 by Hemisphere Publishing Corporation.)
||Pub. in Inhalation Toxicology, v1 n3 p261-271 Jul 89.
|NTIS Title Notes
||Reprint: Effects of Depletion of Ascorbic Acid or Nonprotein Sulfhydryls on the Acute Inhalation Toxicity of Nitrogen Dioxide, Ozone, and Phosgene.
||PC A03/MF A01