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Main Title Constitutive and Stimulated MCP-1, GROalpha, beta, and gamma Expression in Human Airway Epithelium and Bronchoalveolar Macrophages.
Author Becker, S. ; Quay, J. ; Koren, H. S. ; Haskill, J. S. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;TRC Environmental Corp., Chapel Hill, NC. ;North Carolina Univ. at Chapel Hill.
Publisher c1994
Year Published 1994
Report Number EPA/600/J-94/405;
Stock Number PB95-125415
Additional Subjects Cytokines ; Alveolar macrophages ; Bronchi ; Nasal mucosa ; Epithelium ; Polymerase chain reaction ; Tumor necrosis factor ; Growth substances ; Base sequence ; Messenger RNA ; Lipopolysaccharides ; Interleukin-8 ; Reprints ; Monocyte-chemoattractant protein-1 ; GRO proteins
Library Call Number Additional Info Location Last
NTIS  PB95-125415 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 14p
Abstract Constitutive expression of mRNAs for GROalpha, GRObeta, GROgamma, and MCP-1, belonging to the chemokine family of 8-10 kD cytokines with chemotactic properties for granulocytes and monocytes, has been identified in freshly isolated human nasal and bronchial epithelium, and in bronchoalveolar macrophages (AM). Using a semiquantitative RT/PCR technique, the authors found constitutive expression of IL-8 > GROalpha > GROgamma and MCP1, but not GRObeta, in airway epithelial cells. In comparison, AM expressed less GROalpha, similar levels of IL-8, but at least 10 times more GROgamma and MCP1 than the epithelial cells. In addition, AM expressed GRObeta mRNA. Upon reverse transcription, chemokine mRNAs yielded 0.5 to 30 cDNA molecules/cell, depending on the chemokine and cell type. Modulation of chemokine expression by TNFalpha(10 ng/ml) or endotoxin (LPS, 100 ng/ml) exposure was studied in primary nasal epithelial cell and alveolar macrophage cultures. In epithelial cells, LPS did not induce chemokine expression but GROalpha and IL-8 were upregulated approximately 100 fold by TNFalpha; GROgamma expression was elevated 5-10 fold while MCP-1 was increased approximately 40 fold. In AM cultures, all three GROs were strongly induced by LPS with peak mRNA expression 24 h after stimulation. MCP1 mRNA expression, on the other hand, was not induced by LPS. GRO protein was present in supernatants of stimulated epithelial cells and AM while MCP-1 protein was not detectable by western blot anlaysis with a sensitivity limit of 20 ng/ml MCP-1. Together these data emphasize the potential importance of the chemokine family of polypeptides in airway inflammation, as well as the importance of both airway epithelium and AM in these processes.
Supplementary Notes Pub. in American Jnl. of Physiology 266, n3 pL278-L286 Mar 94. Prepared in cooperation with TRC Environmental Corp., Chapel Hill, NC. and North Carolina Univ. at Chapel Hill.
NTIS Title Notes Journal article.
Title Annotations Reprint: Constitutive and Stimulated MCP-1, GROalpha, beta, and gamma Expression in Human Airway Epithelium and Bronchoalveolar Macrophages.
Category Codes 57F
NTIS Prices PC A03/MF A01
Primary Description 600/10
Document Type NT
Cataloging Source NTIS/MT
Control Number 435317916
Origin NTIS
Type CAT