| Main Title |
Glutathione and Mercapturic Acid Conjugations in the Metabolism of Naphthalene and 1-Naphthyl N-Methylcarbamate (Carbaryl). |
| Author |
Chen, Ke-Chang ;
Dorough, H. Wyman ;
|
| CORP Author |
Kentucky Univ., Lexington.;Health Effects Research Lab., Research Triangle Park, NC. |
| Year Published |
1979 |
| Report Number |
EPA-R-805143; EPA-600/J-79-120; |
| Stock Number |
PB81-192049 |
| Additional Subjects |
Glutathione ;
Naphthalene ;
Rats ;
Liver ;
Metabolism ;
In vitro analysis ;
In vivo analysis ;
Carbamates ;
Nitrogen organic compounds ;
Reprints ;
Carbaryl ;
Cysteine/N-acetyl ;
Carbamic acid/naphthyl-N-methyl
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB81-192049 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
23p |
| Abstract |
The formation of glutathione (GSH) conjugate in the detoxification of (1-14C)-naphthalene and (naphthyl-14C)-carbaryl was investigated using rat liver homogenate. The mercapturic acid conjugate in rats was also investigated by collection of urine after intraperitoneal injection of 14C substrates. The formation of water-soluble metabolites in vitro from naphthalene was dependent on the amount of glutathione added, but this was not seen in carbaryl metabolism. In vitro, the metabolism of (1-14C)-naphthalene produced 50% GSH conjugate in the incubation mixture, whereas in vivo the metabolism of this compound produced 65% mercapturic acid conjugate in the urine. There was no evidence of GSH or mercapturic acid conjugate in the metabolism of (naphthyl-14C)-carbaryl in vitro and in vivo. This conclusion was made by comparing the nature and chemical characteristics of GSH and mercapturic acid conjugates formed in (1-14C)-naphthalene metabolism. With the aid of the specific enzyme (e.g. B-glucuronidase and sulfatase) and acid hydrolysis, the water-soluble metabolites of (naphthyl-14C)-carbaryl were tentatively recognized as glucuronide or sulfate conjugated mainly with 5,6-dihydro-5,6-dihydroxycarbaryl or N-hydroxymethyl carbaryl and their hydrolytic products. This data demonstrated that the substituent group on the naphthalene molecule may affect the significance of GSH conjugation. |
| Availability Notes |
Pub. in Drug and Chemical Toxicology, v2 n4 p331-354 1979. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Glutathione and Mercapturic Acid Conjugations in the Metabolism of Nahthalene and 1-Naphthyl N-Methylcarbamate (Carbaryl). |
| PUB Date Free Form |
c1979 |
| Category Codes |
6B; 57A |
| NTIS Prices |
Not available NTIS |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
323797227 |
| Origin |
NTIS |
| Type |
CAT |
