||Structure and Sequence Divergence of Two Archaebacterial Genes.
Cue, D. ;
Beckler, G. S. ;
Reeve, J. N. ;
Konisky, J. ;
||Illinois Univ. at Urbana-Champaign. Dept. of Microbiology. ;Ohio State Univ., Columbus.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.;Department of Energy, Washington, DC.;National Science Foundation, Washington, DC.
||EPA-R-812774, DE-AC02-81ER10945; EPA/600/J-85/545;
Deoxyribonucleic acids ;
Bacterial genes ;
Base sequence ;
Species diversity ;
Methanococcus voltae ;
Methanococcus vannielii ;
Genetic translation ;
Nucleic acid sequence homology ;
||Some EPA libraries have a fiche copy filed under the call number shown.
||The DNA sequences of a region that includes the hisA gene of two related methanogenic archaebacteria, Methanococcus voltae and Methanococcus vannielii, have been compared. Both organisms show a similar genome organization in this region, displaying three open reading frames (ORFs) separated by regions of very high A+T content. Two of the ORFs, including ORFHisA, show significant DNA sequence homology. As might be expected for organisms having a genome that is A+T-rich, there is a high preference for A and U as the third base in codons. Although the regions upstream of the structural genes contain prokaryotic-like promoter sequences, it is not known whether they are recognized as promoters in these archaebacterial cells. A ribosome binding site, G-G-T-G, is located 6 base pairs preceding the ATG translation initiation sequence of both hisA genes. The sequences upstream of the two hisA genes show only limited sequence homology. The M. voltae intergenic region contains four tandemly arranged repetitions of an 11-base-pair sequence, whereas the M. vanielii sequence contains both direct and inverted repetition sequences.
||Pub. in Proceedings of the National Academy of Science USA, v82 p4207-4211 Jun 85. Prepared in cooperation with Ohio State Univ., Columbus. Sponsored by Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab., Department of Energy, Washington, DC., and National Science Foundation, Washington, DC.
|NTIS Title Notes
||Reprint: Structure and Sequence Divergence of Two Archaebacterial Genes.
||57F; 57K; 68G
||PC A02/MF A01