| Main Title |
Homologous Up-Regulation of the Gonadotropin-Releasing Hormone Receptor in alphaT3-1 Cells Is Associated with Unchanged Receptor Messenger RNA (mRNA) Levels and Altered mRNA Activity. |
| Author |
Tsutsumi, M. ;
Laws, S. C. ;
Sealfon, S. C. ;
|
| CORP Author |
Mount Sinai School of Medicine, New York. Dept. of Neurology.;Health Effects Research Lab., Research Triangle Park, NC.;National Science Foundation, Arlington, VA. |
| Publisher |
cDec 93 |
| Year Published |
1993 |
| Report Number |
NSF-91-06877; EPA/600/J-94/324; |
| Stock Number |
PB94-197126 |
| Additional Subjects |
Messenger RNA ;
Up-regulation(Physiology) ;
Gonadarelin ;
Genetic translation ;
Post-transcriptional RNA processing ;
Genetic transcription ;
Northern blotting ;
Pituitary gland ;
Cultured cells ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB94-197126 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
11p |
| Abstract |
The role of altered biosynthesis was investigated in the mouse gonadotrope cell line, by studying the homologous up-regulation of GnRH receptor binding, mRNA levels, and mRNA activity. After GnRH exposure, ligand binding was performed on cell membranes. To correlate changes in receptor number and measurements of biosynthetic activity, GnRH receptor mRNA levels were quantitated by solution hybridization/RNase protection assay and Northern blot analysis, and the capacity of RNA from treated cells to direct the synthesis of new receptors (mRNA activity) was evaluated using a Xenopus oocyte-based bioassay. The authors' results show that although the levels of GnRH receptor mRNA do not vary, treated cells with increased binding capacity show a corresponding increase in cellular GnRH receptor mRNA activity in oocytes. |
| Supplementary Notes |
Pub. in Molecular Endocrinology 7, n12 p1625-1632 Dec 93. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. and National Science Foundation, Arlington, VA. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Homologous Up-Regulation of the Gonadotropin-Releasing Hormone Receptor in alphaT3-1 Cells Is Associated with Unchanged Receptor Messenger RNA (mRNA) Levels and Altered mRNA Activity. |
| Category Codes |
57B; 57F |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
425927214 |
| Origin |
NTIS |
| Type |
CAT |
