Full Record Display for the EPA National Library Catalog


Main Title Generation of Recombinant Baculovirus via Liposome-Mediated Transfection.
Author Hartig, P. ; Cardon, M. ; Kawanishi, C. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Developmental Toxicology Div.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-92/025;
Stock Number PB92-143973
Additional Subjects Baculoviridae ; Genetic recombination ; Liposomes ; Transfection ; Genetic vectors ; Viral DNA ; Plasmids ; Recombinant proteins ; Post-translational protein processing ; Calcium phosphates ; Reprints ; Spodoptera frugiperda
Library Call Number Additional Info Location Last
NTIS  PB92-143973 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 5p
Abstract Baculovirus expression vectors have become a popular method of producing recombinant proteins. Production of recombinant virus requires the transfection of both the native viral DNA and a transfer plasmid into insect cells where recombination takes place. While several methods of transfecting insect cells exist, the authors have found liposome mediated transfection to be the most efficient. The document details the protocols and medium needed for efficient simple transfection of Spodoptera frugiperda cells.
Supplementary Notes Pub. in BioTechniques, v11 n3 p310-313 Sep 91.
NTIS Title Notes Journal article.
Title Annotations Reprint: Generation of Recombinant Baculovirus via Liposome-Mediated Transfection.
Category Codes 57K; 57F
NTIS Prices PC A01/MF A01
Primary Description 600/10
Document Type NT
Cataloging Source NTIS/MT
Control Number 208728778
Origin NTIS
Type CAT