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Main Title Cytochemical Methods for Assessing 'Giardia' Cyst Viability.
Author Lindmark, D. G. ;
CORP Author Cleveland State Univ., OH. Dept. of Biology.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1987
Report Number EPA-R-811949; EPA/600/1-87/011;
Stock Number PB88-124748
Additional Subjects Giardiasis ; Parasitic diseases ; Gastrointestinal diseases ; Epidemiology ; Toxicology ; Air pollution ; Water pollution ; Hazardous wastes ; Cytochemistry ; Toxic substances ; Environmental monitoring
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NTIS  PB88-124748 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 69p
Abstract Giardia lamblia is the most common human intestinal protozoan parasite reported in the United States and England. The objective of the study was to develop a practical, reliable, rapid, microscopically-read method, comparable to excystation for determining the viability of Giardia cysts. At first an attempt was made to use methods dependent on cyst metabolism such as the activity of pyruvate: ferredoxin oxidoreductase (PFOR) and cell respiration. Though they gave promising results with trophozoites, they proved unreliable with cysts. Hence, used was a fluorescent dye exclusion technique previously proved to be successful for measuring viability in mammalian cells.
Supplementary Notes Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
NTIS Title Notes Rept. for 23 Sep 84-22 Dec 86.
PUB Date Free Form Oct 87
Category Codes 57U; 57E; 68A; 68D
NTIS Prices PC A04/MF A01
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 802919722
Origin NTIS
Type CAT