||Isolation of the Alkane Inducible Cytochrome P450 (P450alk) Gene from the Yeast 'Candida tropicalis'.
Sanglard, D. ;
Chen, C. ;
Loper, J. C. ;
||Cincinnati Univ. Medical Center, OH. Dept. of Microbiology and Molecular Genetics.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Cytochrome P-450 ;
Enzyme induction ;
Saccharomyces cerevisiae ;
Fungal DNA ;
Genetic transcription ;
Molecular sequence data ;
Messenger RNA ;
||Some EPA libraries have a fiche copy filed under the call number shown.
||The gene for the alkane-inducible cytochrome P450, P450alk, has been isolated from the yeast Candida tropicalis by immunoscreening a lambda gt11 library. Isolation of the gene has been identified on the basis of its inducibility and partial DNA sequence. Transcripts of this gene were induced by alkane to levels 500 to 1000 fold over those detected in glucose-grown cells. The nucleotide sequence of the 3' portion of this gene revealed a coding sequence for the heme binding segment characteristic of the P450 gene family. (Copyright (c) 1987 Academic Press, Inc.)
||Pub. in Biochemical and Biophysical Research Communications, v144 n1 p251-257 Apr 87. See also PB93-229268 and PB93-229250. Sponsored by Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
|NTIS Title Notes
||Reprint: Isolation of the Alkane Inducible Cytochrome P450 (P450alk) Gene from the Yeast 'Candida tropicalis'.
||PC A02/MF A01