||Isolation of the 'Candida tropicalis' Gene for P450 Lanosterol Demethylase and Its Expression in 'Saccharomyces cerevisiae'.
Chen, C. ;
Turi, T. G. ;
Sanglard, D. ;
Loper, J. C. ;
||Cincinnati Univ. Medical Center, OH. Dept. of Microbiology and Molecular Genetics.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
||EPA-CR-810605 ;EPA-CR-813366; EPA/600/J-93/303;
Cytochrome P-450 ;
Saccharomyces cerevisiae ;
Fungal genes ;
Gene expression ;
Escherichia coli ;
Fungal DNA ;
Drug resistance ;
Molecular sequence data ;
||Some EPA libraries have a fiche copy filed under the call number shown.
||The gene for cytochrome P450 lanosterol 14alpha-demethylase (14DM) from the yeast Candida tropicalis has been isolated. This was accomplished by screening genomic libraries of strain ATCC750 in E. coli using a DNA fragment containing the yeast Saccharomyces cerevisiae 14DM gene. Identity of this gene was confirmed by (a) observing a heme binding region common to all P450s after sequencing the 3' portion of the gene, and (b) based upon tests of its expression in strains of Saccharomyces cerevisiae. (Copyright (c) 1987 Academic Press, Inc.)
||Pub. in Biochemical and Biophysical Research Communications, v146 n3 p1311-1317 Aug 87. See also PB93-229284. Sponsored by Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
|NTIS Title Notes
||Reprint: Isolation of the 'Candida tropicalis' Gene for P450 Lanosterol Demethylase and Its Expression in 'Saccharomyces cerevisiae'.
||PC A02/MF A01